Exploring QTL for Early Heading of Weedy Rice Based on High-density Genetic Map
Journal Title: Journal of Shenyang Agricultural University - Year 2025, Vol 56, Issue 1
Abstract
[Objective]The heading date of rice (Oryza sativa L.) is a quantitative trait regulated by multiple genes, which affects the yield and quality of rice, and determines the planting area and distribution range, heading marks the transformation of rice from vegetative growth to reproductive growth. Weedy rice（Oryza sativa f.spontanea）as a kind of early heading germplasm resources, its heading date is often earlier than that of cultivar, while its genetic mechanism has not been fully elucidated. [Methods]In this study, a recombinant inbred line population consisting of 168 individuals was constructed based on early heading japonica weedy rice WR04-6 and late heading indica rice Qishanzhan. A high-density SNP genetic map was used to apply a inclusive composite interval mapping with additive effect model for QTL mapping at heading stage. [Results]Above the threshold line with a LOD score of 2.5, the only QTL regulating early heading of weedy rice was identified, containing 70 genes in the interval. Based on tissue-specific expression profile analysis and gene annotation, LOC_Os01g08700, also known as OsGI, was speculated as the target candidate gene. Subsequent verification and bioinformatics analysis showed significant differences between different haplotypes of OsGI, which located in the nucleus of the cell and exhibiting significant inter subspecies differentiation between indica and japonica rice. [Conclusion]In the heading regulation pathway of rice OsGI-Hd1-Hd3a/RFT1, Hd1 variation has been widely reported to regulate the heading stage and distribution of rice. However, this study found that the natural variation of the upstream circadian clock gene OsGI in weedy rice may affect its adaptive distribution. This study provides a new perspective on the molecular genetic regulation mechanism of early heading in weedy rice, and also contributes new a targets for the application of early heading gene resources in cultivated rice genetic improvement.
Authors and Affiliations
LI Zhuan, LIANG Chengwei, YU Xiaoyu, HAN Yuqing, CUI Juan, ZHAO Xue, SUN Jian, CHEN Wenfu
Keywords
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- EP ID EP764925
- DOI 10.3969/j.issn.1000-1700.2025.01.001
- Views 16
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