Expression and Purification of the Recombinant OmpL1 Protein for Potential Vaccine Production against Leptospirosis
Journal Title: International Journal of Zoology and Animal Biology - Year 2020, Vol 3, Issue 6
Abstract
Leptospirosis is a popular zoonosis caused by pathogenic strains of the Leptospira. OmpL1 is the outer membrane protein present in most pathogenic Leptospira strains and consider an important factor in the immunogenicity response. We describe here a simple procedure for generating recombinant OmpL1 protein resistant to Leptospira. OmpL1102-316 recombinant was cloned on Escherichia coli strain DH10b and expressed in strain BL21. The purification has performed by an affinity-linked NiSepharose column with His-tag attached to this protein. The recombinant protein OmpL1 induced immunological memory in laboratory rats, suggesting a potential recombinant vaccine production for using in against Leptospirosis in animals.
Authors and Affiliations
Nguyen Tuan Hung, Nguyen Van Huy, Nguyen Duc Hieu, Dang Thi Quynh, Vo Thi Bich Thuy* and Nghiem Ngoc Minh
Keywords
Related Articles
- EP ID EP748216
- DOI 10.23880/izab-16000252
- Views 23
- Downloads 0
Functional Analysis of Growth in Large White Male Turkeys
The Richards and Gompertz functions were used to analyze the growth curves of large white male turkeys. Gompertz function was yt=A*exp (-exp (b-kt)) where; yt is the body weight (g) at age t (weeks), A is asymptotic val...
Interaction of Zinc, Cadmium, Copper, Endosulphan and Dimethoate Pesticides on Fresh Water Fish Channa Punctatus
The aim of the present study to find out the interaction of toxicants and pesticides on fresh water fish Channa Punctatus. We know that chemical mixtures are characteristic of life. The nature of the mixtures varies wid...
Impact of Environmental Pollution on Respiratory System of Human and Animals in Angul and Talcher Industrial Areas, Odisha, India: A Case Study
Industrial growth has been a national issue with leading risk factor towards human health and the environment. The major air pollutants including particle pollution has different toxicological impacts on animals and hum...
Assessment and Monitoring of Microbial Pollution in Certain Drinking and Bathing Water Ponds in and Around Sivagangai
Microbiological examination of water sample is usually undertaken to ensure that the water is safe to drink and bath in. Many potential pathogens could be associated with the water. In this juncture it is very important...
The Birds of Cameroon: The Dynamism of the Cameroonian Avifauna Revealed by Several Bird Surveys in the Centre Region of Cameroon in the Heart of the Congo Basin Forest, Case of Migratory Birds
In this review of several new birds identified in the Centre region of Cameroon, one of the six countries that make up the Congo Basin Forest, we focus on migratory birds. Bird migration appears to be one of the most fa...