EXPRESSION OF ER, PR AND HER2/neu IMMUNOMARKERS IN INFILTRATING DUCT CARCINOMA BREAST IN CELL BLOCK PREPARATIONS AS COMPARED TO HISTOPATHOLOGY
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2018, Vol 7, Issue 31
Abstract
BACKGROUND Fine-Needle Aspiration (FNA) is an easy, quick, inexpensive and reliable technique for diagnosing breast carcinomas. Assessment of Oestrogen Receptor (ER), Progesterone Receptor (PR) and Human Epidermal growth factor Receptor 2 (HER2/neu) expression in breast cancer is necessary for the selection of patients appropriate for hormonal therapy. Hormone receptor study can be done on cytological aspirates rather than on biopsies, thus avoiding a delay in starting treatment. Aim- This study was done to compare immunostaining of these markers in cytology aspirates of infiltrating duct carcinoma breast using cell block preparations with histopathology. MATERIALS AND METHODS Diagnostic test evaluation was done on a study sample of 36 cases of infiltrating duct carcinoma breast received in Department of Pathology, Government Medical College, Kottayam during the study period of 12 months (October 2016 - October 2017). RESULTS The study population had a mean age of 56.17 years. Positivity in cell blocks and histopathology were seen in cases (%): ER, 66.7, 69.4; PR, 52.8, 52.8 and HER2/neu, 16.7, 22.2 respectively. In the assessment of ER, PR and HER2/neu protein (3+) positivity, sensitivity, specificity, Positive Predictive Value (PPV), Negative Predictive Value (NPV) of cell blocks were as follows: ER- 96%, 100%, 100% and 91.67%; PR- 94.73%, 94.11%, 94.73% and 94.11%; HER2/neu- 75%, 100%, 100% and 93.33%. The correlation coefficient between cell block and their corresponding histopathological sections for oestrogen receptor, progesterone receptor and HER2/neu receptor immunostaining were 0.938, 0.889 and 0.837 respectively. CONCLUSION Positivity in cell blocks and histopathology were seen in cases (%): ER, 66.7, 69.4; PR 52.8, 52.8; and HER2/neu, 16.7, 22.2 respectively. There is definite correlation between ER, PR and HER2/neu staining in cell blocks compared to histopathology with a correlation coefficient of 0.938, 0.889 and 0.837 respectively. So, cell blocks can be used for immunostaining, which avoids need for biopsy and delay in starting therapy.
Authors and Affiliations
Ginju Vijayakrishnan, Cicy P. J, Sankar S
Keywords
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- EP ID EP541112
- DOI 10.14260/jemds/2018/794
- Views 75
- Downloads 0
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