Expression of miRNAs confers enhanced tolerance to drought and salt stress in Finger millet (Eleusine coracona)
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2013, Vol 9, Issue 3
Abstract
Plants respond to the environmental cues in various ways, recent knowledge of RNA interference in conferring stress tolerance had become a new hope of developing tolerant varieties. Here we attempt to unfold the molecular mechanism of stress tolerance through miRNA profiling and expression analysis in Finger millet ([i]Eleusine coracona[/i]) under salt and drought stress conditions. The expression analysis of 12 stress specific conserved miRNAs was studied using semi-quantitative real time PCR and Northern blot assay. Our studies revealed that, although most of the miRNAs responded to the stresses, the expression of particular miRNA differed with the nature of stress and the tissue. The expression analysis was correlated with the existing data of their target genes. Abiotic stress up-regulated miRNAs are expected to target negative regulators of stress responses or positive regulators of processes that are inhibited by stresses. On the other hand, stress down-regulated miRNAs may repress the expression of positive regulators and/or stress up-regulated genes. Thus the current study of miRNAs and their targets under abiotic stress conditions displays miRNAs may be good candidates to attribute the stress tolerance in plants by transgenic technology.
Authors and Affiliations
R. Nageshbabu, Usha, M. Jyothi, N. Sharadamma, D. Rai, V. Devaraj
Keywords
Related Articles
- EP ID EP146495
- DOI -
- Views 121
- Downloads 0
EFFECT OF DROUGHT STRESS AND ITS INTERACTION WITH ASCORBATE AND SALICYLIC ACID ON OKRA (Hibiscus esculents L.) GERMINATION AND SEEDLING GROWTH
Effect of drought stress was investigated at water potentials of-0.2 &-0.4 MPA (by using PEG 6000) on okra germination and seedling growth. In this study, percent germination, average time necessary for germination i...
Efficiency of application original preservatives for conservation leukocytes at -40 °C
When frozen leukocytes exposed to harmful factors of the complex, due to their complex cellular structure and high metabolism. Cryopreservatives allow to avoid damages, but most of which are toxic. The aim of the present...
Oxidative Stress Induced Lipid Peroxidation And DNA Adduct Formation In The Pathogenesis Of Multiple Myeloma And Lymphoma
[b]Objective:[/b] To access the oxidative stress status by quantification of byproducts generated during lipid peroxidation and DNA breakdown products generated during DNA damage in the blood serum of multiple myeloma an...
Effect of MgCl2 stress on germination, plant growth, chlorophyll content, proline content and lipid peroxidation in sorghum cultivars
Present report deals with the effect of increasing levels (0-300 mM) of MgCl2 salt on sorghum cultivars, Phule Vasudha and Phule Revati. Although MgCl2 stress did not show considerable adverse effects on germination, how...
ВЛИЯНИЕ БИОТИЧЕСКОГО СТРЕССА НА АКТИВНОСТЬ РАЗЛИЧНЫХ ФОРМ АДЕНИЛАТЦИКЛАЗЫ В ОРГАНЕЛЛАХ КЛЕТОК РАСТЕНИЙ КАРТОФЕЛЯ
Несмотря на значительный интерес, проявляемый к изучению аденилатциклазной сигнальной системы растений, нет полного представления о функционировании и механизмах регуляции данной сигнальной системы у растений при биотиче...