Expression of Proteins Related to Phytophthora capsici Tolerance in Black Pepper (Piper nigrum L.)
Journal Title: International Journal of Agriculture Innovations and Research - Year 2018, Vol 6, Issue 4
Abstract
Vietnam is the number one black pepper exporter in the world. However, pepper production in Vietnam is facing challenges from natural disasters and soil-borne diseases, especially Phytophthora foot rot. Pepper leaves from five black pepper cultivars commonly grown in Vietnam were inoculated with Phytophthora capsici. The activities of pathogenesis-related proteins such as chitinase, β-1, 3-glucanase and phenylalanine ammonia lyase (PAL) significantly increased in the infected leaves compared to the healthy leaves. An SDS-PAGE analysis revealed the expression of two polypeptides with molecular masses of approximately 30 and 10 kDa in the P. capsici-infected leaves after 2 and 3 days following inoculation, respectively. Furthermore, western blot analysis confirmed the presence of a 34 kDa band corresponding to β-1, 3-glucanase in protein extracts from the infected leaves. This study suggests the involvement of β-1, 3-glucanase and related enzymes in the defense response of black pepper to fungal pathogens
Authors and Affiliations
Ton Trang Anh, et al.
Keywords
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