Expression of the Fluoroquinolones Efflux Pump Genes acrA and mdfA in Urinary Escherichia coli Isolates
Journal Title: Polish Journal of Microbiology - Year 2017, Vol 66, Issue 1
Abstract
Escherichia coli is one of the most frequent causes of urinary tract infections. Efflux system overexpression is reported to contribute to E. coli resistance to several antibiotics. Our aim in this study was to investigate the relation between antibiotic resistance and the expression of the efflux pump genes acrA and mdfA in E. coli by real-time reverse transcription-PCR. We tested the in vitro susceptibilities to 12 antibiotics in 28 clinical isolates of E. coli obtained from urine samples. We also determined the minimum inhibitory concentrations of levofloxacin to these samples. We then revealed significant correlations between the overexpression of both mdfA and acrA and MICs of levofloxacin. In conclusion, we demonstrated that the increased expression of efflux pump genes such as mdfA and acrA can lead to levofloxacin resistance in E. coli. These findings contribute to further understanding of the molecular mechanisms of efflux pump systems and how they contribute to antibiotic resistance.
Authors and Affiliations
Sarah M. Abdelhamid, Rania R. Abozahra
Keywords
Related Articles
- EP ID EP225718
- DOI -
- Views 104
- Downloads 0
Intestinal Microbiota, Obesity and Prebiotics
Over the past few decades there has been a significant increase in the prevalence of obesity in both children and adults. Obesity is a disease that has reached epidemic levels on a global scale. The development of obesit...
Polyoma BK virus ( BKPyV) , Epstein-Barr virus (EBV) and Human Papilloma virus (HPV) in oropharyngeal cancer
The aim of this study was to analyze the prevalence of BK virus, Human Papillomavirus and Epstein-Barr virus in oropharyngeal cancer, and to test our hypothesis that BKV/HPV/EBV co-infection plays a role in orophary...
Characterisation of Yersinia Secretion Apparatus - Pathogenicity Island (Ysa-PI) of Yersinia enterocolitica 1B/O8 in Poland: an Idle Ysa Is a Specific Hallmark of the Epidemic Sensu Stricto Strain
[i][/i][i]Yersinia[/i] secretion apparatus (Ysa), the chromosomal type three secretion system (T3SS) is considered to contribute to virulence of high-pathogenicity[i] Yersina enterocolitica[/i] biovar 1B. DNA‑sequen...
<strong>Monitoring of Virulence Genes, Drug-Resistance in <em>Campylobacter coli</em> Isolated from Golden Retrievers</strong>
The investigation was performed on 75 of Golden Retriever puppies. Faecal samples were collected on the 42 day of the puppies life (control). Probiotic preparation was administered on 43 day of the puppies life and 10 da...
<strong>Synthetic Amphibian Peptides and Short Amino-Acids Derivatives Against Planktonic Cells and Mature Biofilm of <em>Providencia stuartii</em> Clinical Strains</strong>
Over the last decade, the growing number of multidrug resistant strains limits the use of many of the currently available chemotherapeutic agents. Furthermore, bacterial biofilm, due to its complex structure, constitutes...