Expression of Toll-like Receptor 2 and 4 in Peripheral Blood Neutrophil Cells from Patients with Chronic Obstructive Pulmonary Disease
Journal Title: Oman Medical Journal - Year 2017, Vol 32, Issue 6
Abstract
Objectives: Chronic obstructive pulmonary disease (COPD) is one of the leading causes of morbidity and mortality around the world. Preliminary studies have evaluated the association between innate immunity including Toll-like receptors (TLRs) and airway samples of patients with COPD. The role of TLRs in peripheral blood neutrophils is poorly understood. Hence, this study aimed to investigate the role of TLR2 and TLR4 in peripheral blood neutrophils of COPD patients. Methods: A total of 101 COPD cases and an equal number of healthy controls participated in this case-control study. Peripheral blood neutrophils were isolated from all participants and cultured for 24 hours through lipopolysaccharide (LPS) stimulation. The gene expressions of TLR2 and TLR4 were assessed by real-time polymerase chain reaction. The protein levels of interleukin (IL)-8 and matrix metalloproteinase (MMP)-9 were measured in neutrophils cell culture supernatants using enzyme-linked immunosorbent assay (ELISA). Results: The levels of IL-8 and MMP-9 were significantly higher in patients with COPD compared to healthy controls. Similarly, the gene expression of TLR2 and TLR4 were increased in LPS stimulated peripheral blood neutrophils of patients with COPD. Smoke pack years was positively correlated with IL-8 levels and negatively correlated with forced expiratory volume in the first second % (r = -0.33; p = 0.023) and FEV1/forced vital capacity (FVC) (r = -0.27; p = 0.011). Conclusions: The increased expression of TLR2 and TLR4 suggests its role in disease pathogenesis of COPD. Smoke pack years was negatively associated with spirometric parameters in COPD patients. This may help to predict the smokers without COPD who risk developing the condition in the future.
Authors and Affiliations
Prashant Mani Tripathi, Surya Kant, Ravi Shanker Yadav, Ram Awadh Singh Kushwaha, Ved Prakash, Sayed Husian Mustafa Rizvi, Arshiya Parveen, Abbas Ali Mahdi, Iqbal Ahmad
Keywords
Related Articles
- EP ID EP259006
- DOI 10.5001/omj.2017.92
- Views 107
- Downloads 0
Tuberculosis in Gulf Health Council Member States: Opportunities and Challenges Towards TB elimination
Tuberculosis (TB) is a major public health problem affecting millions of people globally. In 2016, 10.4 million people developed TB causing 1.7 million deaths. Over 95% of these deaths occurred in low- and middle-income...
The Tip of the Iceberg in Maternally Inherited Diabetes and Deafness
Maternally inherited diabetes and deafness (MIDD) is not only a disorder of the pancreas and ears but a multisystem mitochondrial disorder syndrome. Hypogonadism, however, has not been reported as a phenotypic feature of...
An Unusual Cause of Abdominal Pain in an 8-year-old Girl
An eight-year-old girl presented with a history of fever, nonbilious vomiting, and abdominal pain lasting three days. She was referred from a peripheral hospital for suspected acute appendicitis and admitted to the pedia...
Paget’s Disease in an Omani: Long-term Improvement Following a Single Injection of Zoledronic Acid
Paget’s disease of bone is a patchy skeletal disorder characterized by an increase in bone resorption and formation in the affected areas. It affects up to 3% of individuals of Anglo-Saxon origin over the age of 40 years...
Calcified Ventricular Aneurysm