Expression profile of MYF5 and MYF6 genes in skeletal muscles of young growing gilts of five breeds at different ages, based on the most stable reference genes
Journal Title: Animal Science Papers and Reports - Year 2013, Vol 31, Issue 2
Abstract
The expression profile was evaluated of MYF5 and MYF6 genes in skeletal muscles of young growing Polish Large White (PLW), Polish Landrace (PL), Pietrain (PIE), Duroc (DUR) and Pulawska (PUL) gilts at different ages. Normalization of MYF5 and MYF6 was performed on reliable porcine reference genes (PRGs), where expression stabilities of nine of them (ACTB, B2M, GAPDH, SDHA, HPRTI, RPL13A, YWHAZ, TBP, TOP2B) were evaluated by RT-qPCR method and NormFinder software. Results revealed HPRTI, TBP and TOP2B as highly stable and PRGs. The age-dependent and breed-specific skeletal muscle expression comparisons revealed highly significant (P<0.01) differences in MYF6 expression levels of all skeletal muscles among investigated breeds. MYF6 gene expression in PIE and DUR were higher compared to PLW, PL and PUL gilts. Contrarily, paired-wise comparison of MYF5 gene expression showed only significant difference between DUR and PUL for semimembranosus, and PL and PLW, DUR and PL, PIE and PL, DUR and PUL and PIE and PUL for gluteus medius muscle. There was no significant relationship identified between gilt ages and the level of expression of MYF5 and MYF6 genes. However, their highest expression was identified in longissimus dorsi followed by gluteus medius and semimembranosus muscles. It is concluded that normalization of gene expression has to be done on more than one PRG to reduce the errors in transcription level estimates. Moreover, significantly different breed-specific expression of porcine MYF5 and MYF6 allowed the authors to prioritize these genes as potential candidate genes for trait-associated study.
Authors and Affiliations
Mariusz Pierzchała, Chandra S. Pareek, Paweł Lisowski, Dorota Goluch, Urszula Czarnik, Marian Kamyczek, Ross Gordon Cooper, Jolanta Kurył
Keywords
Related Articles
- EP ID EP71291
- DOI -
- Views 101
- Downloads 0
Polymorphism A/C in exon 7 of the bovine estrogen receptor α (ERα) gene and its association with functional and milk production traits in Red-and-White cattle
Due to the functions that estrogens play in the regulation of reproduction, development of mammary gland, growth and differentiation of cells, estrogen receptors and their genes are considered candidates for molecular ma...
Physiological response to the first saddling and first mounting of horses: comparison of two sympathetic training methods
There is not much research done on the influence of sympathetic training on the emotional reaction of horses. The aim of the present study was to evaluate the emotional response and the stress level in horses to two symp...
The indel polymorphism in cattle amelogenin gene (AMEL) and its significance for the identification and evolutionary studies
Nucleotide and amino acid sequences of cattle amelogenin, the major protein of forming enamel, were analysed by screening 1547 samples collected from cattle of various breeds. Sequenced was the part of cattle exon six of...
The concentration of free fatty acids in goat milk as related to the stage of lactation, age and somatic cell count
The study aimed at determining the concentration of free fatty acids (FFA) and some chemical and physical traits of goat milk as related to the stage of lactation, age and somatic cell count (SCC). Used were 60 Polish Wh...
A single nucleotide polymorphism in CAST gene is associated with meat quality traits in rabbits
The purpose of this research was to investigate the effect of the Calpastatin (CAST) gene on meat quality traits in the Hyla, Champagne and Tianfu Black rabbit breeds. We detected one single nucleotide polymorphism (SNP...