EXPRESSION PROFILING OF SELECTED MICRO RNAs COUPLED WITH MOLECULAR AND BIOCHEMICAL ANALYSES OF ASIATICOSIDES IN CENTELLA ASIATICA (L.)URB IN VITRO
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2019, Vol 11, Issue 5
Abstract
Objective: Centella asiatica (L.) Urb from Umbelliferae is a potential source of secondary metabolites having immense medicinal value. Asiaticoside is the major therapeutic compound. In the present study, Identification of a possible relationship between concentration/transcript level expression of asiaticoside and concentrations of growth hormones at different growth stages was observed. The current study includes molecular and biochemical evaluation of stress generated in C. asiatica at different time intervals in vitro. Methods: The enhancement in auxin, cytokinin and final asiaticoside content were determined using immunoassay kits for auxin, cytokinin and HPLC analysis respectively. Transcript level expression at different growth phases was carried out using real-time RT-PCR. For isolation of stress-related miRNAs, reverse transcription of total RNA using miScript II RT Kit PCR System was carried out as per instructions. The differential expression of five selected miRNAs was done by Real-Time RT-PCR. The analysis of stress in vitro was done by quantification of Hydrogen Peroxide (H2O2), total phenolics and total antioxidants by H2O2 assay kit, total antioxidant assay kit and Folin Ciocalteau reagent respectively. The final asiaticoside content was determined by HPLC. Results: Differential expression of key genes involved in asiaticoside pathway showed significantly higher transcript expression, which is in correlation with the final asiaticoside content. The enhanced expression of miRNAs and the analysis of H2O2, total antioxidant capacity and total phenolics are suggestive of generation of oxidative stress under controlled conditions. Conclusion: The present study shows a direct correlation between oxidative stress and transcript/phytochemical estimation of asiaticoside content under in vitro conditions.
Authors and Affiliations
JISHA S. , HEMANTHAKUMAR A. S. , GOURI P. R. , SABU K. K.
Keywords
Related Articles
- EP ID EP565232
- DOI 10.22159/ijpps.2019v11i5.32097
- Views 151
- Downloads 0
ANTIFUNGAL SCREENING OF 61 TRADITIONAL MEDICINAL PLANTS OF 305 EXTRACTS AGAINST DERMATOPHYTIC FUNGI TRICHOPHYTON TONSURANS
Objective: Antidermatophytic activity of 305 extracts from 61 traditional medicinal plants belonging to 33 different families from Hyderabad Karnataka region was subjected to screening against Trichophyton tonsurans.Meth...
MIR-423-3P USED AS REFERENCE GENE FOR MIRNA 146 A IN CELL LINES HEP-G2
Objective: We explored the stable reference genes for miRNA 146 A by RT-PCR in the cell lines Hep-G2 that were treated chlorogenic acid. We must do a series of tests in order to get a reference genes. Based on the litera...
COMPUTATIONAL STUDY ON THE ANGIOTENSIN CONVERTING ENZYME INHIBITORY POTENTIAL OF THE TEA POLYPHENOLS – CATECHINS: RELEVANCE TO CARDIOVASCULAR DISEASES
Objectives: Secondary metabolites obtained from different plants have been the starting material for designing different drugs. Polyphenols are the secondary metabolites that shows myriad varieties of pharmacological act...
ETHNOZOOLOGICAL REMEDIAL USES BY THE INDIGENOUS INHABITANTS IN ADJOINING AREAS OF THE POBITORA WILDLIFE SANCTUARY, ASSAM, INDIA
Objective: To comprehend the traditional knowledge on zootherapeutic remedies used by indigenous people inhabiting in adjoining areas of the Pobitora wildlife sanctuary located in the Morigaon district of Assam State, In...
STUDY ON VARIATIONS IN THE BRANCHING PATTERN OF ARCH OF AORTA
The most common branching pattern of the aortic arch in humans comprises of three great vessels, during the routine dissection for 1st MBBS we encountered variations in the branches of arch of aorta, so the study was con...