Extended Spectrum β-lactamases and antimicrobial susceptibility among clinical isolates of Pseudomonas aeruginosa in the West Bank, Palestine
Journal Title: Journal of Microbiology and Infectious Diseases - Year 2013, Vol 3, Issue 2
Abstract
Objectives: Class D oxacillinases are frequently acquired by gram negative bacteria in general and P. aeruginosa in particular. P. aeruginosa is commonly implicated in causing nosocomial infections. The evolution of antibiotic resistance in P. aeruginosa and the acquisition of blaOXA genes interfere with successful treatment. Methods: A total of 49 clinical isolates of P. aeruginosa were obtained from Rafidia Hospital, West Bank, Palestine. Antimicrobial susceptibility testing of the isolates was performed by the standard disc diffusion method following the guidelines of CLSI. The prevalence of class D β-lactamases (OXA groups I, II and III) as well as the pseudomonas specific enzymes (CARB-3) were determined by PCR. Results: Susceptibility of P. aeruginosa to carbapenems was the highest 89%, and lowest to ticarcillin/clavulanic acid 70%. This study revealed that P. aeruginosa produced oxacillinase enzymes at rates of: OXA-10 (40.8%), OXA-2 (20.4%) and OXA-1 (18.4%). All ceftazidime resistant strains expressed OXA-1 and OXA-2, 18.4%. PSE group was expressed in 10.2%. Conclusions: This is the first research conducted to investigate the correlation between OXA genes (blaOXA-1, bla- OXA-2 and blaOXA-10) and antimicrobial resistance among P. aeruginosa clinical isolates in Palestine. The results obtained could contribute to better treatment and reduction of the evolution of resistant strains. In addition, it will provide important information regarding the geographical distribution of class D β-lactamases. J Microbiol Infect Dis 2013; 3(2): 56-60 Key words: P. aeruginosa, β-lactamase, susceptibility, oxacillinases, blaOXA genes
Authors and Affiliations
Tamer Essawi, Israr Sabri , Mohammad A. Farraj
Keywords
Related Articles
- EP ID EP136048
- DOI 10.5799/ahinjs.02.2013.02.0081
- Views 104
- Downloads 0
Clonal relation of nasal MRSA carrier status among hospital personnel,hospitalized patients and community
Objectives: Nasal colonization of methicillin-resistant Staphylococcus aureus (MRSA) among the healthcare workers (HCWs), hospitalized patients, and healthy individuals was investigated by pulsed field gel electrophores...
Hearing loss: Can it be neurobrucellosis?
Brucellosis is a zoonotic infection transmitted from animals to humans mostly by ingestion through infected food products, direct contact with an infected animal. It can involve any organ system and present with a b...
Fosfomisinin klinik izolatlara karşı in vitro etkinliği
Amaç: Fosfomisin komplike olmayan üriner sistem enfeksiyonlarının tedavisinde alternative bir ilaçtır. Bu çalışmanın amacı fosfomisinin in vitro etkinliğini, metisiline dirençli Staphylococcus aureus (MRSA), metisiline d...
Multidrug Resistant Bacterial Pathogens in the Indoor Air and Floors of Surgical Wards in a University Teaching Hospital
Multidrug Resistant Bacterial Pathogens in the Indoor Air and Floors of Surgical Wards in a University Teaching Hospital Ahmed Olowo-okere1, Musa Babandina2 1Department of Pharmaceutics and Pharmaceutical Microbiology, U...
First time isolation of Pseudomonas argentinensis from human skin infection
This reported the first isolation of Pseudomonas argentinensis from a human nfection, since the discovery of P. ar gentinensisin 2005. A Palestinian 63-year male practicing gardening on sunny days with exposed arms and l...