Extended Spectrum β-lactamases and antimicrobial susceptibility among clinical isolates of Pseudomonas aeruginosa in the West Bank, Palestine
Journal Title: Journal of Microbiology and Infectious Diseases - Year 2013, Vol 3, Issue 2
Abstract
Objectives: Class D oxacillinases are frequently acquired by gram negative bacteria in general and P. aeruginosa in particular. P. aeruginosa is commonly implicated in causing nosocomial infections. The evolution of antibiotic resistance in P. aeruginosa and the acquisition of blaOXA genes interfere with successful treatment. Methods: A total of 49 clinical isolates of P. aeruginosa were obtained from Rafidia Hospital, West Bank, Palestine. Antimicrobial susceptibility testing of the isolates was performed by the standard disc diffusion method following the guidelines of CLSI. The prevalence of class D β-lactamases (OXA groups I, II and III) as well as the pseudomonas specific enzymes (CARB-3) were determined by PCR. Results: Susceptibility of P. aeruginosa to carbapenems was the highest 89%, and lowest to ticarcillin/clavulanic acid 70%. This study revealed that P. aeruginosa produced oxacillinase enzymes at rates of: OXA-10 (40.8%), OXA-2 (20.4%) and OXA-1 (18.4%). All ceftazidime resistant strains expressed OXA-1 and OXA-2, 18.4%. PSE group was expressed in 10.2%. Conclusions: This is the first research conducted to investigate the correlation between OXA genes (blaOXA-1, bla- OXA-2 and blaOXA-10) and antimicrobial resistance among P. aeruginosa clinical isolates in Palestine. The results obtained could contribute to better treatment and reduction of the evolution of resistant strains. In addition, it will provide important information regarding the geographical distribution of class D β-lactamases. J Microbiol Infect Dis 2013; 3(2): 56-60 Key words: P. aeruginosa, β-lactamase, susceptibility, oxacillinases, blaOXA genes
Authors and Affiliations
Tamer Essawi, Israr Sabri , Mohammad A. Farraj
Keywords
Related Articles
- EP ID EP136048
- DOI 10.5799/ahinjs.02.2013.02.0081
- Views 112
- Downloads 0
Mide kanserinde Helicobacter pylori ile DNA hipermetilasyonunun ilişkisi
Mide kanseri (MK) tüm dünyada sık görülen kanserlerden biridir. MK oluşumu genetik ve epigenetik değişikliklerin birikimi ile çok adımlı ilerleme gösterir. Deoksiribonükleik asit (DNA) dizisi değişmeden gen ifadesinde me...
The prevalence of HIV and the role of immigration in Albania
Objective: International migration is an increasingly important aspect of global, regional and national economies. The rapid spread of HIV among communities, states and continents, is the evidence of the link betwee...
Staphylococcus lugdunensis bacteraemia secondary to an infected intracardiac fragment of a vascular access device
We report a case of Staphylococcus lugdunensis bacteraemia following infection of a fragmented totally implantable vascular access device. This report highlights the virulence of S. lugdunensis especially in immunosuppre...
Crimean-Congo hemorrhagic fever and pregnancy: Two cases
Crimean-Congo Hemorrhagic Fever (CCHF) is a viral zoonosis, transmitted to humans by either: the Hyalomma species of ticks; or by direct contact with body fluids or tissues of infected humans or domestic animals. CCHF c...
Nasal Extranodal Natural Killer/T Cell Lymphoma in a 69-Year Old Female: A Case Report
Mature natural killer /T-cell (NK/T-cell) lymphomas are a rarely occurring subgroup of Non Hodgkin Lymphomas (NHL). A large majority of NK/T cell lymphomas are extranodal. Nasal type is the most common one. As clinical s...