Extended Spectrum β-lactamases in Clinical Isolates of Escherichia coli and Enterobacter cloacae Collected from Nablus District - Palestine
Journal Title: Microbiology Research Journal International - Year 2016, Vol 16, Issue 3
Abstract
Aim: To determine the prevalence of extended-spectrum β-lactamases (ESBLs) among clinical isolates of Escherichia coli (E. coli) and Enterobacter cloacae (E. cloacae) in Nablus district. Methodology: In this prospective study carried out at An-Najah National University, a total of 161 bacterial isolates were collected during a12-month period in Nablus district in Palestine. To detect ESBLs, the isolates were examined by combination disc method. PCR was used to detect blaCTX-M, blaTEM and blaSHV genes in 32 representative ESBL-producer E. coli isolates. Results: Using combination disc method, ESBL enzymes were detected in 73 out 153 (47.7%) E. coli and in 1 out of 8 (12.5%) E. cloacae isolates. No significant association of ESBL-producer E. coli was observed with types of collected specimens, gender, hospital ward, outpatient, or medical source. Among 32 representative E. coli ESBL-positive, blaCTX-M, blaTEM and blaSHV genes were detected in 30 (93.8%), 2 (6.3%) and 1 (3.1%), respectively. Two new variants of ESBLs (PALTEM137b and PALSHV-2a') were identified. A unique E. cloacae isolate expressing inducible class C B-lactamase was also detected. Conclusions: In Nablus region, high frequencies of ESBLs were found among E. coli bacteria isolated from outpatients and inpatients. blaCTX-M is the predominant gene among ESBL producers. New variants of ESBLs were found.
Authors and Affiliations
Motasem Al-Masri, Nael Abu-Hasan, Maha M. Jouhari
Keywords
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- EP ID EP350867
- DOI 10.9734/BMRJ/2016/27892
- Views 95
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