Extracellular Enzymatic Assays of Lipolytic Bacteria on Lipid-Rich Abattoir Waste water
Journal Title: Haya: The Saudi Journal of Life Sciences - Year 2018, Vol 3, Issue 2
Abstract
Abstract:Wastewater from abattoirs has great impact on the environments, since it is made up of a significant portion of organic contents such as lipids containing high BOD and COD. Amongst other sources of lipid-rich wastewater, in the abattoir wastewater, a significant amount of bacterial capable of utilizing lipids and other organic contents has been found. Exploiting the extracellular hydrolytic capabilities of these microbes is necessary for the overall reduction in the organic contents of the wastewater. Lipolytic bacterial cultures of Pseudomonas aeruginosa, Staphylococcus aureus,Klebsiellapneumoniae, and Bacillus subtilis were used in the treatment of the lipid-rich wastewater with enrichment nutrient combinations. Thus, the extracellular enzymes; lipase, protease and amylase were assayed at 6hour intervals for a period of 48hours for hydrolytic activity. Bacillus subtilis treatment showed the highest amylase and protease activity while Pseudomonas aeruginosa showed the highest lipase activity and high amylase and protease activities with Staphylococcus aureus, also a good lipase producer. The result indicated extracellular production of the enzymes to cater for the initial breakdown of substrates in the wastewater medium. Owing to the enzymatic activities of lipase, protease and amylase possessed by these lipolytic organisms, they could prove to be beneficial for bringing down the overall organic load of abattoir wastewater. Keywords:Abattoir, Wastewater, Lipids, Enzymes, Lipolytic bacteria, Degradation, Hydrolytic, Extracellular.
Authors and Affiliations
Ononiwu Prosper Ikechukwu, Ekwenye, Uchechi N
Keywords
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