EXTRACTION, BIOACTIVITIES, PHYTOCHEMICAL INVESTIGATION AND IN-VIVO TOXICITY STUDIES OF MESUA FERREA L. STAMENS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 4
Abstract
Objective: To investigate in-vitro antibacterial and free radical scavenging potentials of Mesua ferrea L. stamens which are used in traditional medicinal preparations along with their phytochemical investigation and in-vivo toxicity studiesMethods: Various extracts of M. ferrea stamens were prepared by kinetic maceration method using four organic solvents. Potent antibacterial n-hexane extract of stamens was selected in the preliminary screening for antibacterial activity which was performed by an agar diffusion method for further studies. Quantification of antibacterial activity of n-hexane extract was carried out using broth microdilution method as per CLSI guidelines. Phytochemical investigations of the same extract were performed using qualitative tests for the detection of various phytochemical groups and Libermann-Burchard colorimetric assay for determination of total terpenoid content. In-vivo safety of the extract was determined by acute oral toxicity studies in mice as per OECD guidelines test no.420.Results: n-hexane extract of M. ferrea stamens was found most potent amongst other extracts studied for antibacterial activity; moreover it exhibited bactericidal activity against selected bacterial pathogens. The same extract exhibited good free radical scavenging activity in DPPH assay (IC50 value = 66.3 µg/ml). Phytochemical investigation of extract revealed presence of sterols, terpenoids and volatile oil components and the total terpenoid content was estimated as 102.8 mg/ml of dried extract (in terms of lupeol equivalents). The extract was found safe in mice in acute oral toxicity studies.Conclusion: M. ferrea stamens exhibited broad spectrum antibacterial activity along with good free radical scavenging potential and in-vivo safety which encourage further studies in the area.Â
Authors and Affiliations
Komal D Barbade, Ajit G Datar
Keywords
Related Articles
- EP ID EP578851
- DOI -
- Views 65
- Downloads 0
NEUROPROTECTIVE EFFECTS OF POMEGRANATE JUICE ON LEAD INDUCED NEUROTOXICITY IN MICE
Article Withdraw On Author's Request
A ROLE FOR PHALERIA MACROCARPA (SCHEFF) BOERL. EXTRACTS IN THE MANAGEMENT OF WOMEN’S PATHOLOGICAL CONDITIONS: A RESEARCH REVIEW
Phaleria macrocarpa (Scheff) Boerl is a medicinal plant that originates from West Papua, Indonesia. The fruit of this plant is known to contain numerous different compounds that produce different bioactivities. Many of t...
SEROPREVALENCE AND GENOTYPING OF HEPATITIS C VIRUS AMONG HOSPITAL BASED GENERAL POPULATION IN VIJAYPUR, INDIA
Objective: Hepatitis C virus (HCV) has emerged as a leading cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. According to World Health Organization (WHO) there are 180 million people affected wit...
EFFECT OF NON-VOLATILE SOLVENT AND EXCIPIENT RATIO ON FLOW AND CONSOLIDATION PROPERTIES OF POWDER BLEND FOR LIQUISOLID COMPACTS
Objective: To study the effect of non-volatile solvent and excipient ratio on flow and consolidation properties of powder blend for liquisolid compacts.Methods: The effect of non-volatile solvent and excipient ratio on f...
MEMBERSHIP RATE, PERCEPTION AND AWARENESS OF THEIR PROFESSIONAL ASSOCIATION AMONG NEWLY LICENSED PHARMACISTS
Objectives: The Malaysian Pharmaceutical Society (MPS) is the professional association for pharmacists in Malaysia. It plays a significant role in the continuous education of pharmacists by serving as a platform to infor...