Extraction of lipids and purification of linoleic acid from Clarias macrocephalus oi
Journal Title: Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society - Year 2011, Vol 4, Issue 3
Abstract
Fatty acid composition of “keli” (Clarias macrocephalus) oil has been shown to contain 18.4% of linoleic acid (LA). Oil was obtained from three types of extraction methods namely Blight & Dryer method, Soxhlet method, and pressing method. Blight & Dryer method produced an acceptable total lipid content with good quality of oil. After saponification and acidification, purification of released LA by urea complex with urea to fatty acid ratio 5:1 and crystallization temperature of 20°C, had enhanced purity of LA to 47% with total fatty acid recovery of 25.2%. The LA obtained was converted to LA methyl ester prior to further purification by argentated silica gel chromatography. LA with a purity of 92% with total fatty acid recovery of 12.9% was obtained.
Authors and Affiliations
Noorul Zainuddin, Abdul Babji, Mamot Said
Keywords
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