Extrusion-Based Bioprinting through Glucose-Mediated Enzymatic Hydrogelation
Journal Title: International Journal of Bioprinting - Year 2020, Vol 6, Issue 1
Abstract
We report an extrusion-based bioprinting approach, in which stabilization of extruded bioink is achieved through horseradish peroxidase (HRP)-catalyzed cross-linking consuming hydrogen peroxide (H2O2) supplied from HRP and glucose. The bioinks containing living cells, HRP, glucose, alginate possessing phenolic hydroxyl (Ph) groups, and cellulose nanofiber were extruded to fabricate 3D hydrogel constructs. Lattice- and human nose-shaped 3D constructs were successfully printed and showed good stability in cell culture medium for over a week. Mouse 10T1/2 fibroblasts enclosed in the printed constructs remained viable after 7 days of culture. It was also able to switch a non-cell-adhesive surface of the printed construct to celladhesive surface for culturing cells on it through a subsequent cross-linking of gelatin possessing Ph moieties. These results demonstrate the possibility of utilizing the presented cross-linking method for 3D bioprinting.
Authors and Affiliations
Enkhtuul Gantumur, Masaki Nakahata, Masaru Kojima and Shinji Sakai
Keywords
Related Articles
- EP ID EP678724
- DOI -
- Views 159
- Downloads 0
Coaxial nozzle-assisted electrohydrodynamic printing for microscale 3D cell-laden constructs
Cell printing has found wide applications in biomedical fields due to its unique capability in fabricating living tissue constructs with precise control over cell arrangements. However, it is still challenging to print c...
A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl
3D bioprinting is an emerging technology that enables the fabrication of three-dimensional organised cellular constructs. One of the major challenges in 3D bioprinting is to develop a material to meet the harsh requireme...
A methodology to develop a vascular geometry for in vitro cell culture using additive manufacturing
Today, additive manufacturing (AM) is implemented in medical industry and profoundly revolutionizes this area. This approach consists of producing parts by additions of layers of successive materials and offers advantage...
Concentric bioprinting of alginate-based tubular constructs using multi-nozzle extrusion-based technique
Bioprinting is a layer-by-layer additive fabrication technique for making three-dimensional (3D) tissue and organ constructs using biological products. The capability to fabricate 3D tubular structure in free-form or ver...
The mussel-inspired assisted apatite mineralized on PolyJet material for artificial bone scaffold
With the development of three-dimensional (3D) printing, many commercial 3D printing materials have been applied in the fields of biomedicine and medical. MED610 is a clear, biocompatible PolyJet material that is medical...