F GENE SEQUENCING AND ANALYSIS OF INDIAN NEW CASTLE DISEASE VIRUS
Journal Title: International Journal of Agriculture Sciences - Year 2017, Vol 9, Issue 17
Abstract
Newcastle disease (ND) is one of the most important infectious diseases of poultry. Incidences of ND in vaccinated chicken population of Anand area of Gujarat demanded a study for pathotyping and genetic characterization of the field NDV isolates. Ten allantoic fluid samples from the SPF eggs inoculated with NDV suspected tissue suspension were used for the RNA isolation, followed by one step RT-PCR. All the ten isolates yielded expected 362bp product of F gene indicating them positive for NDV. Multiple sequence alignment of Anand isolates with reference Indian Bareilly strain and two vaccine strains R2B and LaSota revealed 7 unique mutations in Anand isolates. The amino acid sequences of all the ten Anand isolates showed RRQKRF between 112 and 117 positions indicating them to be of virulent type. NDV isolates showed maximum genetic similarity 94.32% with genotype XIII strain (Chicken/Sweden/97) indicating isolates to be of genotype XIII.
Authors and Affiliations
P. D. RABAR, M. K. JHALA, B. B. BHANDERI, R. A. MATHAKIYA
Keywords
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