Fast track method for the identification of multi-drug resistant tuberculosis on direct clinical specimen using combined drug media
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2011, Vol 1, Issue 1
Abstract
Objective: To combine isoniazid (INH) and rifampicin (RIF) in a single media to detect and evaluate multi-drug resistant Mycobacterium tuberculosis (MDR-TB) strains using clinical specimens by direct and indirect drug susceptibility testing (DST). Methods: Drug susceptibility testing for INH and RIF using individual and combined drug media was performed by minimum inhibitory concentration (MIC) method on direct clinical specimens. Results: The combined drug media showed complete concordance with individual drug media in the detection of MDR-TB by direct DST method and 89% efficiency with indirect DST method. Susceptibility results were available by 3 weeks after the receipt of clinical specimen using direct DST on combined drug media. Conclusions: Combined drug media can be used as a fast track method in large scale studies warranting detection of MDR-TB.
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- EP ID EP234481
- DOI 10.1016/S2222-1808(11)60011-X
- Views 108
- Downloads 0
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