FEATURES OF ASSOCIATIVE LINKS BETWEEN NONHOMOLOGOUS ALLELES OF THE GENE-ENZYME SYSTEMS IN DIFFERENT RAPANA GROUPS OF BLACK SEA
Journal Title: Вісник Одеського національного університету. Біологія - Year 2016, Vol 21, Issue 1
Abstract
Rapa whelk (Rapana venosa, Valenciennes, 1846) has a great influence on the operation of such an isolated marine ecosystem, as the Black Sea. However, many aspects of its vital functioning, including the genetic and biochemical mechanisms of Rapana high adaptive capacity, practically unknown. Therefore, the aim of this work was to find among Rapana genotypes stable combinations (pairings, association) of different alleles of a gene-enzyme systems, the totality of which can be regarded as adaptive gene complexes. The shellfish from waters of the three north-western parts of the Black Sea (Odessa Bay, Tarhankut and island Zmeiniy) were investigated. There were analyzed 169 individuals of a species R. venosa. The homogenates for electrophoretic separation were prepared from gland of Leiblein of molluscs; the identification of allozymes were performed in PAA-gels. The following enzymes were studied: alkaline phosphatase, NADH oxidase, esterase, alcohol dehydrogenase and glutathione peroxidase. The eight loci, including two for next enzimes - alkaline phosphatase, esterase and NADH oxidase were chosen for the testing of genotypes; the alcohol dehydrogenase and glutathione peroxidase were presented by one locus for each enzime. In conducting the genetic analysis based on the hypothesis about the two allel structure of the investigated loci. The presence of conjugation between alleles of different loci, controlling different enzyme systems, determined by the coefficient of association and Russell-Rao coefficient of linkage disequilibrium. It revealed that between certain alleles of enzymes' loci formed temporary association. Specific association of alleles in a population can be kept for several years; this fact may indicate their adaptive value. The example of such combination is a long association between alleles of alkaline phosphatase Alp-126 and the alcohol dehydrogenase Adh03. Number of associated alleles of the gene-enzyme systems to a certain Rapana group due to the general state of the animals in the waters: the largest number of complexes between alleles of gene-enzymes systems were observed in a set of Odessa Bay Rapana individuals (grouping in good condition), the lowest - in Tarkhankut waters (grouping in the doldrums). It is suggested that the role of the alleles in the adaptation of associations: negomologinih found temporary combinations of alleles can act as a source of adaptive gene complexes. It is suggested that the role of the alleles associations is important for the adaptation : detected temporary combination of non-homologous alleles can act as a source of adaptive complexes.
Authors and Affiliations
V. A. Toptikov, V. M. Totskyi, T. H. Alekseeva, O. O. Kovtun
Keywords
Related Articles
- EP ID EP308684
- DOI -
- Views 81
- Downloads 0
FISH FAUNA RESEARCH RESULTS RELATED TO ODESA BAY COASTAL MARINE AREA IN COURSE OF 2016–2017
Research purpose: monitoring the coastal fish community condition in Odesa bay during 2016-2017. Materials. 2868 fish individuals from 55 samples screened during two years. Methods. Fish has been sampled using stake gil...
SELECTIVITY IN FEEDING OF RAPA WHELK RAPANA VENOSA (VALENCIENNES, 1846) BY MUSSELS MYTILUS GALLOPROVINCIALIS LAMARCK, 1819
Feeding selectivity of rapa whelk, Rapana venosa (Valenciennes, 1846) on mussels Mytilus galloprovincialis Lamarck,1819 was estimated by the caging experiment in the coastal waters of the Zmeinii Island (north-western Bl...
FRUCTIFICATION AND FORM DIVERSITY OF PETIOLATE OAK (QUERCUS ROBUR L.) OF THE NATURAL AND ARTIFICIAL SOUTH EAST OAK WOODS OF UKRAINE
Introduction. In recent decades, degradation and mass drying of oak forests to a greater or lesser degree have been observed in many European countries. Popula tions of Q. robur in the Donetsk region are on the southern...
DYNAMICS OF THE CONTAMINATIONS WITH FEATHER MITES (ACARI: ASTIGMATA) OF BLACKCAP AND ROBIN MIGRATING THROUGH ZMIINYI ISLAND (THE BLACK SEA, UKRAINE).
Problem. The contamination of the plumage of the Blackcap Sylvia atricapilla (Sylviidae) and the Robin Erithacus rubecula (Muscicapidae), migrating through Zmiinyi island (the Black Sea, Ukraine) with feather mites with...
IDENTIFICATION AND CHARACHTERIZATION OF ACTIVE AGAINST FUNGOUS MIDGE BRADISIA PILISTRIATA FRAY STRAINS OF BACILLUS SP. ONU15 AND BACILLUS SP. ONU29
By the methods of FAMES analysis, multiplex PCR with group-specific primers, phase-contrast microscopy, electron microscopy, plasmids profile detection, SDS-PAGE, gradient ultracentrifugation and RAPD with M13 Bacillus s...