Ferric Chelate Enhances Azo Dye Decolourisation by Crude Horseradish Peroxidase
Journal Title: Universal Journal of Environmental Research and Technology - Year 2014, Vol 4, Issue 6
Abstract
The mechanisms of oxidation of azo dyes by the Fenton-like process and the enzyme Horseradish peroxidase are similar. Both processes occur between pH 2.0-4.0. Horseradish peroxidase is a heme containing enzyme. Ferric-Ethylenediamine tetraacetic acid (Ferric-EDTA) which resembles the chelated Ferric ion of the heme group was selected as an additive for the decolourisation of three azo dyes- Methyl Orange, Bismarck Brown and Tartrazine by Horse peroxidase. The decolourisation was computed using the difference between initial and final absorbance of the dye solutions over 24 hours. It was found in the present study, that the addition of Ferric-EDTA improved the decolourisation of Methyl Orange and Bismarck Brown at pH 6.0 and Tartrazine at pH 9.0; by 10.33%, 13.41% and 1.58%, respectively. The enhanced decolourisation may be because FerricEDTA mimics the oxidative decolourisation action of peroxidase on the tested azo dyes. The activity of crude peroxidase was enhanced using 0.23 mmol L-1 and 0.56 mmol L-1 at pH 6.0 and 9.0 respectively. Methyl Orange and Bismarck Brown are decolourised at relatively mildly acidic condition, pH 6.0 as against pH 2.0- 4.0 in presence of the additive. The findings of the present study are significant in the enzymatic decolourisation of wastewater containing azo dyes. Enzymatic wastewater treatment requires large amounts of enzymes. An additive that can mimic the action of the enzyme and enhance its performance can reduce the total amount of enzyme used in the treatment. This may even help to reduce the running cost of enzymatic wastewater treatment.
Authors and Affiliations
Mugdha Ambatkara and Usha Mukundana
Keywords
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