Ferulic acid modulates ultraviolet-B radiation mediated inflammatory signaling in human dermal fibroblasts
Journal Title: Journal of Research in Biology - Year 2014, Vol 4, Issue 8
Abstract
Ultraviolet B (UVB 290-320 nm) participate in the development of the cutaneous inflammatory response which includes a cascade of events that involves increased expression of cyclooxygenase-2 (COX-2), release of tumor necrosis factor-alpha (TNF-α) and other inflammatory cytokines. Peroxisome proliferator-activated receptors (PPARα/γ) are considered to be potential targets for photo protection because they inhibit UVB mediated inflammatory responses. In this study, we investigated the effect of ferulic acid on UVB-radiation induced expression of TNF-α and COX-2 in human dermal fibroblasts (HDFa). Further, the action of ferulic acid on PPARα/γ activation and its binding interaction with these proteins were analyzed by induced fit docking. We found that onetime UVB exposure (19.8 mJ/cm2) showed significantly increased the expressions of COX-2 and TNF-α in HDFa after 4 h post-irradiation when compared to the control cells. Ferulic acid pretreatment for 30 min before UVB exposure prevented UVB-induced overexpression of these inflammatory markers. It has also been found that ferulic acid activates PPARα/γ expressions in HDFa. Further, induced fit docking analysis showed that there was a greater binding interaction of ferulic acid with PPARγ than PPARα. Thus, ferulic acid exhibits beneficial effects against UVB-induced inflammatory responses probably through down-regulating COX-2 and TNF-α expressions and activating PPARα/γ agonists
Authors and Affiliations
Kanagalakshmi A, Agilan B, Mohana S, Ananthakrishnan D, Velmurugan D, Karthikeyan R, Ganesan M, Srithar G, Rajendra Prasad N
Keywords
Related Articles
- EP ID EP567232
- DOI -
- Views 71
- Downloads 0
Study on display sequences in Cotton Pygmy-Goose Nettapus Coromandelianus Coromandelianus Gmelin
The pairing and display sequence of Cotton Pygmy-goose (Nettapus coromandelianus coromandelianus Gmelin) in Eastern Assam was observed from April 2007 to July 2008. The frequency of head movement (up-down and left-right)...
Puntius viridis (Cypriniformes, Cyprinidae), a new fish species from Kerala, India
Taxonomic analysis of eight specimens of a cyprinid fish collected from Manimala River, Kerala, India revealed that they present several morphological differences from their congeners. The new species, Puntius viridis, i...
Identification of dust storm sources area using Ackerman index in Kermanshah province, Iran
Dust storms are one of the most important environmental challenges in the last decade at Kermanshah province, which greatly endangers the health of all living things, especially humans. The first step in controlling this...
Efficient methods for fast, producible, C-Phycocyanin from Thermosynechococcus elongatus
This article describes different protocols that enhance the extraction, isolation and purification of phycocyanin from the cyanobacterium, Thermosynechococcus elongatus as well as absorbance and fluorescence spectral cha...
Investigation of volatile organic pollutants in atmospheric air in Tehran and acetylene pollutants in Tehran qanat waters
The United Nations General Assembly interprets the issue of air pollution and its consequences as a common concern of humanity, and the growing development of an international flow calls for immediate and decisive action...