Fibrin Rich in Leukocyte-Platelets (L-PRF) and Injectable Fibrin Rich Platelets (i-PRF), Two Opportunity in Regenerative Surgery: Review of The Sciences and Literature.
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2019, Vol 18, Issue 4
Abstract
PRF is a second-generation platelet concentrate and can be distinguished in P-PRF (Pure Plateletrich fibrin) and L-PRF (Leukocyte and Platelet-rich fibrin). An evolution of L-PRF is the injectable PRF (iPRF). In vivo, during the platelet clot formation, platelets are bound to β-integrin fibrin and the clot retracts itself. The PRF clot forms a solid fibrin matrix, displaying a complex tridimensional architecture. Measuring the levels of PDGF-BB, TGF-1β and IGF-1, in the PRF matrix, it was revealed that slow fibrin polymerization during PRF elaboration leads to the intrinsic platelet formation of cytokines and glycan chains inside the fibrin mesh. The PRF proximal part (the part closer to the red clot) contains platelets and leukocytes, found to be massively enclosed inside the fibrin net. Platelet localization inside PRF was examined through immunostaining and scanning electron microscope (SEM). Compared to the abrupt basal compression of PRF membrane (GPRF), preservation of plasma level, of 3D fibrin, and of platelets was found to be more intact in PRF membrane preparations obtained through metallic compression system (PRF-C) (L-PRF Wound Box). The pure plateletrich fibrin (P-PRF) and the leukocyte and platelet-rich fibrin (PRF-L) are biomaterials with solid fibrin or biomaterials containing leukocytes. No platelet concentration related problems were found in PRF, as all platelets in the drawn blood sample are activated and integrated inside the fibrin matrix of the clot. Approximately 97% of platelets and more than 50% of leukocytes are concentrated inside the PRF clot, which displays a specific tridimensional distribution. Almost all platelets (>97%) are absent inside test-tubes of groups tested after PRF membrane extraction. Factors freed by platelets contained in L-PRF induce and control the proliferation and migration of other cell types, involved in tissue repair, like smooth cell muscles (SMCs) and mesenchymal stem cells (MSCs). This review article focuses on the development of various platelet concentrates, their fabrication procedure, advantages and disadvantages for use in regenerative surgery and healing process.
Authors and Affiliations
Alessandro Crisci, Silvana Manfredi, Michela Crisci
Keywords
Related Articles
- EP ID EP573537
- DOI 10.9790/0853-1804106679.
- Views 61
- Downloads 0
Incidence and Clinical Profile of Triple Negative Breast Cancer (TNBC)
Objective: To study the incidence and clinical profile of triple negative breast cancer (TNBC) patients.MethodsPatients with histopathological evidence of breast cancer were evaluated for ER, PR HER-2 neu by immunohistoc...
Curve Of Spee in Orthodontic
Curve of Spee is a naturally occurring phenomenon in the human dentition. This normal occlusal curvature is required for an efficient masticatory system. Orthodontists eventually deal with the curve of Spee in virtually...
The cause and duration of delay in medical examination of victims of sexual offence in a tertiary care setting of a metro city in India
In our society there is significant underreporting of cases of sexual offence. Even if a case is reported, it is already late. And when the medical examination of the victim is done more time elapses. Thus important find...
Cytological Evaluation of Thyroid Lesions and its Correlation with Histopathology: A Study in a Rural Tertiary Care Hospital, Konkan belt of Maharashtra, India.Cytological Evaluation of Thyroid Lesions and its Correlation with Histopathology: A Study in a Rural Tertiary Care Hospital, Konkan belt of Maharashtra, India.
Background: Thyroid diseases are common problem of enormous magnitude now a day all over the world. And it is necessary in the clinical practice is to distinguish reliably the few malignant thyroid tumors from the harmle...
Impression Techniques in Implant Dentistry
Dental implants are rapidly gaining popularity in its use as fixed prosthesis. Implant impression is one of the most important steps in achieving passive fit by accurately relating an analogue of the implant or implant a...