Fibroblast Growth Stimulation, DPPH Antioxidant Assay and Antimicrobial Activities of Funtumia elastica (Preuss) Stapf (Apocynaceae) Leaf Extracts
Journal Title: European Journal of Medicinal Plants - Year 2014, Vol 4, Issue 7
Abstract
Aims: To investigate the scientific basis for the wound-healing properties of Funtumia elastica (Apocynaceae) leaf extracts using relevant in vitro fibroblast growth stimulation, antimicrobial and DPPH-antioxidant assays. Place and Duration of Study: School of Health, Sports and Bioscience (Bioscience Laboratories), University of East London in the United Kingdom, between July 2007 and May 2010. Methodology: Methanolic extract of the leaves, and petroleum ether, ethyl acetate, n-butanol and aqueous fractions partitioned thereof were tested for antimicrobial activities against common wound pathogens (such as Staphylococcus spp, Pseudomonas aeroginosa and Escherichia coli). The Broth dilution method was used to determine the minimum inhibitory concentrations (MIC) of the extracts and fractions. The antioxidant activities were also determined using a 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay; whilst the ability to stimulate fibroblast growth was investigated using the MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay. Results: The n-butanol fraction exhibited the greatest overall activities. It stimulated the growth of fibroblast cells by 28%, and showed MIC range of 0.13 - 1.0 mg/mL against the Staphylococci species, P. aeruginosa, Bacillus subtilis and E. coli. The non-polar petroleum ether fraction exhibited MICs greater than 2.0 mg/mL against all the organisms. All the fractions exhibited antioxidant activities greater than or comparable to that of ascorbic acid. Conclusion: Collectively, the antioxidant activity, fibroblast growth stimulation and the antimicrobial activities demonstrated by F. elastica leaf extracts provide some evidence to support the use of the plant to manage wounds in African folklore medicine.
Authors and Affiliations
Samuel N. Osei-Djarbeng, Sally J. Cutler, Ronald R. Cutler, Olivia Corcoran
Keywords
Related Articles
- EP ID EP350041
- DOI 10.9734/EJMP/2014/9316
- Views 76
- Downloads 0
HPLC/MS/MS Study of Phenolic Compounds of Leucaena leucocephala Legumes Monitored with Their in vitro Antihyperglycemic Activity
High pressure liquid chromatography coupled with mass spectrometry (HPLC/MS/MS) is considered as one of most the sensitive modern techniques used for determination of phenolic compounds, hence in this research it was uti...
Anti-inflammatory Activity of Leaf Extract and Fractions of Tapinanthus bangwensis (Engl. & K.Krause) Danser Parasitic on Citrus angustifolia
Aim: This study was undertaken to evaluate the anti-inflammatory activity of extract of the leaves of the plant. Study Design: The study adopted the experimental design. Place and Duration of Study: Department of Pharmac...
Cytotoxicity of the Aqueous Extract of Clerodendrum splendens on HaCaT Keratinocytes
Clerodendrum splendens G. Don (Family Verbenaceae) has been used for centuries to treat various medicinal problems in Africa, including the treatment of wounds and other skin conditions. Though several studies have been...
Antihypercholesterolemic, Cardioprotective and Vitamins E and C Sparing Properties of Bryophyllum pinnatum in Rabbits
Potential of Bryophyllum pinnatum in preventing excessive lipaemia and oxidation, hence reducing predisposition to chronic diseases was assessed. B. pinnatum supplement was prepared by macerating a known weight of the pl...
Phytochemicals, Nutritional Analysis and In vitro Antioxidant Activities of Pickled Perilla frutescens Ethanolic Leaf Extract
Aims: With pickled perilla leaves as raw materials, this paper proposed the optimal ethanol extraction conditions and made a profound analysis for extract in the compositions of major active ingredients, nutrients, miner...