Fibroblastic phenotype in oral submucous fibrosis- a cell culture analysis
Journal Title: Saudi Journal of Pathology and Microbiology - Year 2017, Vol 2, Issue 3
Abstract
Abstract: Arecoline present in the arecanut permeates through the oral mucosa and induces increase in the collagen production and decrease in the collagen degradation. This indicates the probability of a different phenotype of fibroblast being operative in the causation of oral submucous fibrosis (OSF). The present study aimed to establish cell lines of fibroblast from normal buccal mucosa and OSF tissues of human, identify the various morphological forms of fibroblasts; understand and assess the response of the fibroblast cell lines to different concentrations of arecoline. fibroblast cell lines were obtained from control samples as well as from OSF. The cell lines were treated with 50/100/150/300/500 ug/ml of arecoline from control and cases. The response of the cell lines were evaluated over a period of 8 days on the basis of cell counts. The fibroblast cell lines of the control and the cases exhibited different morphological forms i.e.F1 spindle, F2 epitheloid and the F3 stellate form. The F3 to F1 ratio was higher in OSF. There was dose dependent response observed in arecoline treated cell lines. The untreated cell lines showed an increase in the cell counts over the period of 8 days and the control fibroblasts showed a higher proliferation. It was found that arecoline at 50ug/ml was slight stimulatory and at 150ug/ml it was found to be cytotoxic to the cell lines. Arecoline can be stimulatory or enhance proliferation of the fibroblast at lower concentrations but acted as cytotoxic at higher concentrations. This is because of the response of the arecoline receptors at various concentrations of the arecoline. Keywords: OSF, fibroblast, cell lines, arecoline.
Authors and Affiliations
Abhishek Banerjee, V. V Kamath, Vijayalakshmi Kotrashetti, Kishore Bhatt
Keywords
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