Field Performance and Gene Expression of Drought Stress Tolerance in Cotton (Gossypium barbadense L.)
Journal Title: Biotechnology Journal International - Year 2016, Vol 14, Issue 2
Abstract
Twenty cotton genotypes belonging to Gossypium barbadense L. were used to screen the cotton genotypes for drought stress tolerance and to study gene expression of some genes related to cotton tolerance for drought stress. According to the field performance, highly significant genetic differences were found between cotton well-watered and water-stressed treatments as well as among the twenty cotton genotypes. According to Drought Stress Index (DSI), the genotypes “10229”, “Suvin” and “Giza75” were most drought tolerant. Meanwhile, the genotypes “Giza92”, “[Giza84x(Giza70xGiza51B)xs6]” and “Giza93” were the drought moderate tolerant. On the other hand, the most drought susceptible genotype was “Giza85”. The above mentioned genotypes were used to study the gene expression of different genes related to drought stress tolerance in cotton. The gene flowering locus T-like protein 1 (FTL1) and the Gossypium heat shock protein 1 (GHSP1) have been expressed in most genotypes (with different degrees of intensity) except in the pattern of genotype “Giza85” (control and stressed) which suggest that these genes clearly discriminate the drought susceptible cotton genotypes. The general trend of the expression of Lea2A and MFT1 genes was not affected by the drought stress. No discriminate trend was obtained for the genes Lea4 D9a and Lea4 D9b, whereas, different band patterns were noted. It can be said that the most affected genes with drought stress are FTL1 and HSP1 genes and can be used to determine the drought susceptible cotton genotypes.
Authors and Affiliations
Kamal F. Abdellatif, Hesham M. Hamoud, Yasser A. M. Soliman, Samah M. M. Eldemery
Keywords
Related Articles
- EP ID EP237974
- DOI 10.9734/BBJ/2016/26643
- Views 110
- Downloads 0
Towards Efficient In vitro Regeneration of Cowpea (Vigna unguiculata L. Walp): A Review
Cowpea is a crop of tremendous economic and ecological values particularly in sub-Saharan Africa, where over 80% of the crop is produced and consumed. Due to heavy attack by pests and diseases, actual yield does not exce...
Biovalorization of Olive Mill Waste Water for the Production of Single Cell Protein from Saccharomyces cerevisiae, Candida utilis and Pleurotus ostreatus
Aims: The aim of this work was to investigate and optimize the potential of olive mill waste water (OMWW) to be utilized as a substrate for the production of single cell protein (SCP). Study Design: The study was divided...
Effect of Ad-libitum, Split and Restricted Feeding on Performance, Digestibility and Welfare of Broiler Chickens
An experiment was conducted to assess the performance, welfare and digestibility of broilers fed varying forms of feeding regimes. The objective of this work research was investigate the possibility of improving growth p...
Characterization of Spent Bleaching Earth and Its Utilization for Improving Manure-based Biogas Production
Aim of the Study: To utilize spent bleaching earth (SBE) to improve biogas production efficiency through co-digesting with manure and provide a practical way of disposing the SBE. Design of the Study: The SBE was collec...
Characteristics of Penicillin G Acylase Immobilized onto Iron Oxide Nanoparticles
Penicillin G acylase was immobilized onto iron oxide nanoparticles coated with polyethyleneimine and then cross linked with glutaraldehyde solution. The FTIR spectrum of immobilized enzyme showed peak at 1648cm-1 which c...