FimH Typing to Investigate Sub Clone Variations in ST69, ST127 and ST131 of Uropathogenic E. coli (UPEC) Isolates
Journal Title: International Journal of Medical and Health Sciences - Year 2016, Vol 5, Issue 2
Abstract
Introduction: Multilocus sequence typing (MLST) used to characterize Uropathogenic Escherichia coli (UPEC) is not discriminatory enough because strains with identical sequence types (ST) may possess different genotypes which enable different life- styles. Therefore there is a need for a typing scheme with superior discriminatory power between closely related strains. The aim of this study is to provide a typing scheme for UPEC ST69, ST127 and ST131 which will provide superior discriminatory power among them. Materials and methods: Sequences of FimH genes detected in 41 UPEC isolates recovered from patients with Urinary tract infection at Central Manchester University hospital between 2007-2011 were used. Their MLST grouping already showed that 20 were of ST131, 10 were of ST127 and 11 were of ST69. Using their fimH sequences and reference sequence of E.coli fimH K-12, consensus sequences were formed. These consensus sequences were aligned to fumC and an alignment created. Pair wise comparison was carried out for phylogenetic tree construction. Molecular revolutionary relationships were examined by the neighbor-joining method. Results: The isolates were found to have originated from the same ancestral lineage. Every isolate in each ST was closely related to one another as indicated by the clusters they formed which consisted primarily of their respective STs. Conclusion: FimH and fumC sequence analysis used in typing UPEC isolates is useful in investigating and understanding the fine population structure of UPEC. It cannot represent standard MLST but when used in parallel with MLST, it gives higher discriminatory result than MLST alone.
Authors and Affiliations
Agi Vivian Nkemkanma
Keywords
Related Articles
- EP ID EP39951
- DOI -
- Views 322
- Downloads 0
Double toothed mandibular third molar – A rare anomaly – case report
Abberantmorphoanatomy of tooth are not uncommon, like fusion,gemination, concresence, dilacerations, talons cusp, dens in dente, dens evagenatus, taurodontismand supernumerary tooth. But gemination or fusion of mandibul...
Effect of Structured Mentorship on New Entrant Medical Students
Aim : To deliver structured mentorship to first year medical students and to scientifically document its effect on their scholastic performance. Materials & Methods : In a prospective randomised control trial, first yea...
Fused multicystic kidneys with Persistent Cloaca – Case report
Renal anomaly with Urorectal septum malformation (URSM) with persistent cloaca is a very rare congenital anomaly. During dissection of 22 weeks old spontaneously aborted male fetus in the department of Anatomy, We found...
Precaval Right Renal Artery: Is It More Common?
Background: Thorough knowledge of renal vascular variations has major clinical implications as it contributes to the safe performance of surgical and interventional procedures and proper interpretation of radiological i...
Histological Changes in the Placentae from Severe Anaemic Mothers
The information provided from the pathological assessment of the placenta may provide important clinical information for both the mother and the neonate. Anaemia in pregnancy is a well recognized obstetric hazard, obse...