Fine structure of Somatotrophs in Pars Distalis of the Indian wild Caught Female Bat, Taphozous nudiventris kachhensis (Dobson)
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2017, Vol 1, Issue 2
Abstract
The ultra structural observations on Somatotrophs During estrus cells are spherical to oval in shape with the spherical nucleus. Cytoplasm of cell is filled with a large number of round to oval shaped secretory granules of diameter ranges from 200-400nm. Cells during early pregnancy are polyhedral in shape with eccentric nucleus and mostly euchromatic. Mitochondria are spherical to oval in shape with lamellar cristae. The Golgi zone is observed juxtanuclear in position. Secretory granules are increases in number as compared to estrus. STH cell during lactation are polyhedral in shape. Rough endoplasmic reticulums are elongated, tubular dotted with ribosomes. Secretory granules are seen scattered in the cytoplasm. The diameter of secretory granules is about 230 nm. The light and ultra structural findings on the pituitary gland by many workers gave much more information about different cell type in pituitary gland. The different cell found in the pars distalis play very important role in the reproductive cycle of the animals. Biologists attempting to investigate central endocrine mechanisms in bats face obvious difficulties on all these fronts. Wild-caught bats, sampled at different phases of their reproductive cycles, have provided the basis for most of our current knowledge. As a result, most studies have been descriptive in nature, documenting apparent changes in hypothalamic and pituitary activity that occurs over the course of male and female reproductive cycles. However, efforts to maintain healthy captive bat colonies, in which individuals not only survive over the long term, but also reproduce, are becoming increasingly successful. Nerkar, Gadegone [1] have reported the structure of FSH cell in pars distalis of female bat Taphozous longimanus during different phases of reproductive cycle. STH cell are identified and differentiated on the basis of activity of Golgi body, and the elaboration of secretory granules, and the liberation of granules from cell membrane and morphology of mitochondria. Beside this shape and size of cell and nucleus. In Vespertilionid bat, Scotophilus heathi Singh, Krishna [2] have reported STH cell in pars distalis on the basis of specific morphological characters, staining reactions and immune characteristics. The detailed structure of STH cell in pars distalis of female bat Hipposideros lankadiva have been studied by Seraphim [3]. The present study was designed to elucidate the ultra structural changes in STH cell of the pars distalis of female bat, T. kachhensis during the physiology of reproduction. T. kachhensis (Dobson) is an exclusive Indian Emballonurid bat found in caves, tunnels and temples. The bat selected for present study because of unique habits. The specimen of Taphozous kachhensis were collected from Aambai Nimbai, 45 kilometers from Bramhapuri (M.S.). Many collections were made during the breeding season so as to coincide with the time of reproductive cycle and to get an accurate pregnancy record. For the electron-microscopic study, the intermediate and posterior lobes were separated from the pituitary gland and the isolated anterior pituitary was fixed in fresh icecold 3% glutaraldehyde for three hours and then four hours in 0.1 M cacodylate buffer. The tissues were washed in buffer and then post fixed for one to two hours in 1% 0.067M cacodylatebuffered osmium tetroxide. After dehydration with graded series of alcohol, the tissues were cleared in propylene oxide solution and embedded in Araldite resin which would be polymerized at 60ºC. Then, ultrathin sections from selected blocks were cut with glass knife and picked up on 400-mesh copper grids. Sections were double stained with 10% alcoholic uranyl acetate for 20 min and for 10 min in Reynold’s lead citrate. The sections were examined under a JEM Jeol-100s electron microscope (Japan) at 80KU accelerating voltage and photographed. The current concept of cell identification and differentiation in the mammalian pituitary gland is based on the activity of the Golgi apparatus, the elaboration of secretory granules, libration of granules from the cell membrane, development of the ergastoplasm and morphology of the mitochondria. Beside this, the shape and size of cell, nucleus and the distribution and morphological feature of secretary granules are also taken in to consideration. All these constitute visible changes which allow us to determine the state of secretory activity of each of the adenohypophysial cell. The present ultra structural observation demonstrates the presence of six types of cells in the pars distalis of non-pregnant bat, pregnant and lactating bats of T. kachhensis.
Authors and Affiliations
Pankaj R Chavhan
Keywords
Related Articles
- EP ID EP569171
- DOI 10.26717/BJSTR.2017.01.000227
- Views 131
- Downloads 0
Results of Vitamin D Prescription in High Schools
Introduction: Online Medicare is a method in which parts of a medical process - whether its diagnostics, monitoring or the treatment itself will be done by using online services. This system has been operated in 4 boys h...
Efficient Expansion of Human Umbilical Cord Blood- Derived NK Cells Ex Vivo without Requiring Feeder Layers
Natural killer (NK) cells are central components of the innate immunity. They have ability to kill a wide range of cancer cells and are a promising tool for both autologous and allogeneic immune enhancement therapy in ca...
Chemotherapy Safety Standards in the Developing World: is G CSF the Only Option?
The developing world poses a new set of challenges for the ever more demanding field of oncology. Not only that one has to cope with the concept of new treatments, there are different patient expectations and difficultie...
Assessment of Microbial Contamination if “As Received”and “Bench-Top Exposed” Orthodontic Materials: A VitroMicrobiologic Investigation
Introduction: The assumption of sterility of packed materials used in orthodontics may lead practitioners to use them without the necessary sterilization. Hence, this study was undertaken to assess the microbial contamin...
Prognosis Comparison Molecular Subtype Breast Cancer between Young and Adult Women at General Hospital Dr. M Djamil Padang
Breast cancer is a heterogenic disease with various biologic profiles and clinical prognosis. A research in Netherland by Esther, et al 2013, showed that molecular subtypes of breast cancer have different distribution an...