First approach to molecular epidemiology of bovine tuberculosis in Colombia
Journal Title: Revista MVZ Cordoba - Year 2016, Vol 21, Issue 1
Abstract
Objective. To investigate the presence of Mycobacterium bovis and other Mycobacterium species in samples of cattle and buffalo in Colombia, to start the molecular characterization of M. bovis in the country. Material and methods. 492 samples were collected from herds identified with the presence of infected animals through the PPD, by the Group of Bovine Tuberculosis ICA Colombian Agricultural Institute in eight departments of Colombia. Lymph nodes of head, thorax and abdomen, gross lesions of tissues with tuberculosis, nasal swabs, milk, blood and fresh cheeses were included. Samples were subjected to detection of Mycobacterium bovis and other mycobacteria by conventional microbiological analysis and PCR-6110 and spoligotyping molecular assays. Results. In the samples analyzed especially in lymph nodes, Mycobacterium bovis was demonstrated with genotypes reported and not previously reported in the world, as well as M. tuberculosis in Antioquia, Cundinamarca, Boyacá and Magdalena departments. Conclusions. In Colombia there are at least 7 genotypes of M. bovis that are infected and sick cattle and buffalo from four different departments becoming serious threat to public health.
Authors and Affiliations
Jimena Jojoa-Jojoa, M. Sc, Maira Wintaco M, M. Sc| Instituto Nacional de Salud, Dirección de Investigación en Salud Pública, Grupo de Micobacterias, Avenida Calle 26 # 51-20 Bogotá D.C. Colombia., Francisco Osorio R, MV| Instituto Colombiano Agropecuario ICA, Programa de Brucelosis y Tuberculosis bovina, Carrera 41 #17-81 Bogotá D.C. Colombia., Gloria Puerto-Castro, M. Sc *| Instituto Nacional de Salud, Dirección de Investigación en Salud Pública, Grupo de Micobacterias, Avenida Calle 26 # 51-20 Bogotá D.C. Colombia.Correspondencia: gpuerto@ins.gov.co, Martha Guerrero-Guerrero, Bact| Instituto Nacional de Salud, Dirección de Investigación en Salud Pública, Grupo de Micobacterias, Avenida Calle 26 # 51-20 Bogotá D.C. Colombia.
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