First molecular evidence of Toxoplasma gondii in opossums (Didelphis virginiana) from Yucatan, Mexico
Journal Title: Open Veterinary Journal - Year 2016, Vol 6, Issue 1
Abstract
Toxoplasma gondii is an obligate intracellular parasite recognized as a causal agent of toxoplasmosis; zoonotic disease endemic in many countries worldwide, including Mexico. Different species of animals participate in the wild cycle infection, including opossums of the species Didelphis virginiana. Thirteen D. virginiana were captured in Yucatan, Mexico. Detection of T. gondii was achieved by Polymerase Chain Reaction, which determined an infection of 76.9% (10/13) in brains. Positive amplicons were sequenced for analysis, this produced results similar to T. gondii with identity and coverage values of 98% and 96-100%, respectively. This study presents the first molecular evidence of the circulation of T. gondii in D. virginiana from Mexico.
Authors and Affiliations
M. Torres-Castro| Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, Universidad Autónoma de Yucatán, Mérida, México, H. Noh-Pech| Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, Universidad Autónoma de Yucatán, Mérida, México, R. Puerto-Hernández| Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, Universidad Autónoma de Yucatán, Mérida, México, B. Reyes-Hernández| Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, Universidad Autónoma de Yucatán, Mérida, México, A. Panti-May| PIMVS, Tumbel Kuxtal A.C., Tixkokob, México, S. Hernández-Betancourt| Departamento de Zoología, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Mérida, México, A. Yeh-Gorocica| Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, Universidad Autónoma de Yucatán, Mérida, México, L. González-Herrera| Laboratorio de Genética, Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, Universidad Autónoma de Yucatán, Mérida, México, J. Zavala-Castro| Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, Universidad Autónoma de Yucatán, Mérida, México, F. I. Puerto| Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, Universidad Autónoma de Yucatán, Mérida, México
Keywords
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