First Report of Banana Septoria Leaf Spot Disease Caused by Septoria eumusae in Iran
Journal Title: International Journal of Farming and Allied Sciences - Year 2014, Vol 3, Issue 11
Abstract
Septoria leaf spot is one of the fungal diseases of banana leaves that has been reported in Southern and Southeast Asia. In year 2011, an undescribed leaf spot disease of banana was discovered in Southeast Iran. Symptoms of disease were observed in the Hormozgan, Sistan & Beluchistan areas. Symptoms in the initiation of infection appear as small necrotic, lentis shape and dark brown spots similar Sigatoka symptoms, which soon later enlarge rapidly and become rot. In this research, samples, that showed symptoms of Septoria leaf spot were collected from banana orchards in Chabahar, Konarak, Minab and Jask areas and were transferred to laboratory. Leaf samples were first washed and were surface sterilized with 1% sodium hypochlorate and then diseased sections with necrosis were cut into 2×2cm sections. They were stapled to a sterile filter paper and placed in the lid of petri dish on water agar (WA) medium. The obtained spores were transferred to potato dextrose agar (PDA) medium. Pathogenisity tests were carried out with selected isolations on using detached leaves of Harichal cultivar. In this research, fungus, Septoria eumusae (Anamorph Mycosphaerella eumusae) was isolated from infected tissues. The results showed that, all fungi isolate created symptoms in inoculated leaves and then reisolated from those. This is the first record of Septoria leaf spot disease in Iran. At present, it is a disease of major important affecting banana in Iran.
Authors and Affiliations
M. Amani, Gayane Avagyan
Keywords
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