Flow Cytometry Analysis of Peripheral Blood B Cell Distribution of Patients with Multiple Sclerosis
Journal Title: UNKNOWN - Year 2017, Vol 23, Issue 4
Abstract
Objective: Multiple sclerosis (MS) is a central nervous system (CNS) disease characterized by autoimmune inflammation and neurodegeneration. Damage to the CNS is thought to be mediated predominantly by activated pro-inflammatory T cells and antibody secreting B cells. Strong evidence of B cell functions in MS pathogenesis has come from trials of B cell- depleting treatment. In this study, the peripheral blood frequencies of B cell subsets were measured using flow cytometry in patients to determine the disease-specific B cell differences that might be associated with the evolution to progressive forms of MS. Materials and Methods: Peripheral blood mononuclear cells were separated from patients and healthy controls [relapsing-remitting MS (RRMS) and secondary progressive MS (SPMS)]. Cells were stained with anti-human monoclonal antibodies (CD19-APC, CD27-FITC, IgD-APC/Cy7, CD138-PE, CD24-PerCP and CD38-Alexa fluor 700), and analyzed using flow cytometry Results: There were no significant differences between the MS group and healthy controls by means of peripheral blood frequencies of B cells, immature, naïve, classic memory, plasma, plasmablasts, and regulatory B cells. Only higher naïve B cell frequency tendency was determined in patients with RRMS as compared with patients with SPMS and healthy controls. Conclusion: Peripheral blood B cell subset measurements are not likely to be used as a biomarker for prediction of disease progression. Although B cells have a well-known pathogenic significance, B cell population alterations do not occur during the progression of the disease.
Authors and Affiliations
Vuslat Yılmaz, Deniz Ak Tura, Canan Ulusoy, Duygu Özkan Yaşargün, Suzan Adın Çınar, Recai Türkoğlu
Keywords
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- EP ID EP276844
- DOI 10.4274/tnd.87523
- Views 63
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