Fluconazole susceptibility of Candida species isolated from cases of Vulvovaginal Candidiasis by using E-test method
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2017, Vol 6, Issue 3
Abstract
Candida vaginitis is one of the most frequently encountered forms of superficial candidiasis. The principal agent of vulvovaginal candidiasis is C.albicans, but other species known generally as C.nonalbicans (C.glabrata, C.tropicalis, C.krusei, C.parapsilosis, C.lusitaniae) are also isolated. The available therapeutic arsenal for treatment of vulvovaginal candidiasis offer few options and includes the imidazole and trizole agents, in topical and oral form. The E-test has been introduced as an easier testing procedure and an alternative for the NCCLS method. The present study determines the distribution of Candida species and in vitro susceptibilities of fluconazole against the Candida isolated from vulvovaginitis candidiasis using E-test. Methods: Two vaginal swabs from each patient were processed by microscopy and culture to know the prevalence of candidialvulvovaginitis and to study their antifungal susceptibility pattern of fluconazole by E-test. Results: Out of 300 suspected cases of vulvovaginitis, only Candida as a cause of vulvovaginitis was present in 133(44.33%) cases. The most common age group associated with vulvovaginal candidiasis was 20-29 years (43.61%) followed by 30-39 years (30.83%) and greater than 40 years (25.56%). The most common species isolated was C.albicans followed by C.glabrata. By E-test method 92.21% of C.albicans, 60.46% of C.glabrata and all (100%) C.tropicalis was susceptible, that is, showing MIC of fluconazole less than or equal to 8μg/ml and all 100% C.krusei was resistant (MIC≥64μg/ml) to fluconazole. Conclusion: For effective treatment of the infection, to prescribe the correct medication and to overcome recurrence, it may be advisable to identify the Candida species routinely from vaginal swabs of infected women. Also, the high growing rate of resistance of non-albicansCandidato azoles confirms the importance of monitoring changes in the distribution of pathogenic Candida species. The major feature of E-test method is that it can allow a quick answer concerning Candida resistance to antifungal agents, preventing unnecessary patients’ drug abuse.
Authors and Affiliations
Dr. Poornima Dhandale| Departmentof Microbiology, Government Medical College, Nagpur, Maharashtra, India., Dr. Seema P Khetan| Department of Microbiology, Government Medical College, Nagpur,Maharashtra, India., Dr. M S Qazi| Department of Microbiology, Government Medical College, Nagpur, Maharashtra,India
Keywords
Related Articles
- EP ID EP3857
- DOI -
- Views 385
- Downloads 23
Carbon Nanotube/Polypyrrole Composites as a Novel Solid Phase Microextraction Fiber Coating for the Preconcentration of n-Alkanes from Aqueous Media
In this paper, the electrochemical fiber coating (EFC) technique was used for the preparation of oxidized multiwalled carbon nanotubes–polypyrrole composite (MWCNTs–PPy) and applied as a new fiber for solid-phase micro...
POTENTIAL INFLUENCE OF CULTURE MEDIUM CONSISTENCE AND SILVER NITRATE ON MICROPROPAGATION OF SOLANUM VIARUM, AN ANTICANCER MEDICINAL PLANT
An understanding of optimal nutrient concentration could lead to increased growth and also could evoke maximum morphogenesis in vitro more effectively. Experiments were conducted to determine suitable combination of AgNO...
A Review on Nanocochleate – A Novel Lipid Based Drug Delivery System.
Nanaocochleate represent a new approach for oral and systemic delivery of drugs . It is a novel lipid-based system which is suitable for the oral and systemic administration of a wide variety of molecules with important...
Recent Advances in Novel Semisolid Dosage Forms: An Overview
Semisolids constitute a significant proportion of pharmaceutical dosage forms. They serve as carriers for drugs that are topically delivered by way of the skin, cornea, rectal tissue, nasal mucosa, vagina, buccal tissue,...
INFLUENCE OF NON SURGICAL THERAPY ON SERUM ALBUMIN AND WBC COUNT IN DIABETIC AND NON DIABETICS WITH CHRONIC PERIODONTITIS
BACKGROUND: Periodontal disease is a complex, multifactorial, chronic inflammatory disease that involves destruction of Periodontium. Many systemic diseases have been implicated as risk factors or risk indicators in peri...