FLUORESCENCE ANALYSIS OF SAMPLE OF SHEEP TO ESTABLISH THE PRESENCE OF BACTERIUM PASTEURELLA MULTOCIDA THE CAUSE OF DANGEROUS DISEASE IN LARGE AND SMALL RUMINANTS
Journal Title: Engineering and Technology Journal - Year 2016, Vol 1, Issue 4
Abstract
Field method for the study of herd animals was developed. This method allows the analyzing of samples from animals where the farm is. It does not require any special conditions. Therefore, it is widely applicable to the study of both large and small ruminants. The field method does not harm the animal or the operator who is working with the appliance. The experimental set up was used to characterize the samples of ruminants in the field test samples by means of a UV light source. What is needed is just a small sample from the body of the animal that fluoresces and depending on the emission wavelengths bacteria or other illegal substances can be detected. Samples taken from a sheep that suffered from Pasteurellosis disease show the presence of the bacterium Pasteurella multocida. Using UV and fluorescence spectroscopy we have studied spectral wavelength, except for Pasteurella multocida and for β-defensin, lipopigment lipid, infusoria, pepsin, P-glycoprotein and peptides. The device is applicable to both large and small herd animals. It will help farmers with fast and accurate diagnosis of ruminants to solve global problems for both livestock breeding and their products for the food industry.
Authors and Affiliations
Vanya Plachkova, Chavdar Zemyarski, Petar Petrov, Marin Nenchev, Margarita Deneva
Keywords
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