FORMS OF PRESERVATION OF SOME BOTANICAL ELEMENTS: DIANTHUS SEROTINUS WALDST & KIT., VAR. TRANSILVANICUS NOVÁK
Journal Title: Natural Resources and Sustainable Development - Year 2013, Vol 3, Issue 1
Abstract
Preservation through in vitro multiplication of the specie Dianthus serotinus Waldst & Kit., var. transilvanicus Novák, was followed, a specie critically threatened (CR), a Dacian-Pannonian endemism, a species from the country’s flora, also important for its ornamental value. At the initiation of the in vitro culture we started from the juvenile flower bud, gathered from the field from Alba County (Râpa Roşie, Lancrăm) from young plants at the first flowering. Explants were cultivated on Murashige Skoog (1972) medium with the following variants: Do = MS; D1 = Do + 3g/l CV + 1g/l EGP; D2 = Do + 0,1mg/l AIB + 0,1mg/l BA + 825mg/l NH4NO3; D3 = Do + 0,1mg/l AIB + 2mg/l BA + 40mg/l AdSO4, which after three months of in vitro culture evolved depending on the composition of the medium. The best evolution took place on D1 (economical medium devoid of phytohormones), on which there were obtained completely conformed plants. On the variants with phytohormones and additional additives there were reported undesired phenomena, vitrification or hyperhidricity of the neoplantlets due to the additional additive of 825 mg/l NH4NO3; or the differentiation of a weak Radicular System (short roots and few in number), but a large number of neoplantlets, about 85 neoplantlets/explant, due to the presence of BA and of AdSO4 (D3). Completely conformed neoplantlets (Photo. 1) acclimatized in a percentage of 80%, in compliance with the stages of acclimatization (Laslo, 2013). During the first year they were kept in greenhouses or in cold seedbeds, then in the spring of the second year they were planted in a definitive place (Cluj-Napoca Botanical Garden, Râpa Roşie Reserve, Alba County), a year in which they gained forcefulness. We believe that form of ex situ conservation through in vitro multiplication is in the pipeline and also it is of interest for the conservators and for the species threatened with extinction and which also have an ornamental value. For Dianthus serotinus Waldst & Kit., var. transilvanicus Novák, sozological, being critically endangered specie (CR), Dacian-Pannonian geoelement and endemism, in vitro micropropagation technique makes possible its conservation in a botanical garden, in a reserve or in its area of origin, following periodically the capacity to adapt of the new plantlets at their in vitro transfer.
Authors and Affiliations
Vasile Laslo, Maria Zăpârțan, Eliza Maria Agud
Keywords
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