Free Radical Scavenging Activity and HPLC Analysis of Araucaria cunninghamii Aiton ex D. Don Leaf Extract
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2014, Vol 10, Issue 3
Abstract
[b]Background:[/b] Several diseases are caused in the body due to oxidative stress of free radicals. The objectives of the present study were to investigate the antioxidant activity of [i]Araucaria cunninghamii[/i] Aiton ex D. Don ([i]Araucariaceae[/i]) leaf extract. The dried leaves of [i]Araucaria cunninghamii[/i] Aiton ex D. Don ([i]Araucariaceae[/i]) were extracted with 80% methanol. The antioxidant activity of the extract was predicted through in vitro model systems such as 1,1-diphenyl-2-picryl hydrazyl (DPPH), reducing power and nitric oxide scavenging assays. The total phenolic, flavonoid and tannin content of the extract were also estimated in order to draw the correlation with their bioactivities. The extract was also investigated for several polyphenolic compounds using High-performance liquid chromatography (HPLC). [b]Results:[/b] The antioxidant activity of extract was found to be excellent in all the three assays. A positive correlation between the antioxidant activity and phenolic content was observed. HPLC analysis identified the presence of Gallic acid, Catechin, Chlorogenic acid, Epicatechin, Caffeic acid, Umbelliferone, Ellagic acid, Quercetin and Kaempferol in the methanol leaf extract of [i]Araucaria cunninghamii [/i]Aiton ex D. Don. [b]Conclusions:[/b] The results of the present study point towards the fact that [i]Araucaria cunninghamii [/i]Aiton ex D. Don leaves possess good antioxidant potential. The strong antioxidant activity can be correlated with the polyphenolic compounds present in the leaves.
Authors and Affiliations
Vandana Gautam, Saroj Arora, Dhriti Kapoor, Renu Bhardwaj
Keywords
Related Articles
- EP ID EP152630
- DOI -
- Views 88
- Downloads 0
ДЕТЕКЦИЯ ВЛИЯНИЯ ОБРАБОТКИ АНАЛОГАМИ ПРЕПАРАТА «СИЛК» РАСТЕНИЙ КАРТОФЕЛЯ В ПОЛЕВЫХ УСЛОВИЯХ
Хвойные экстракты, такие как Новосил и Лариксин в небольших дозах (0.3 мл/л) повышают стрессоустойчивость растений картофеля на примере изменения активности пероксидазы и в чистом виде подавляют рост возбудителя кольцево...
Cold Hardening Prevents H2O2-induced Programmed Cell Death in Maize Coleoptiles
An influence of cold hardening (8 °С, 7 days) on the respiration intensity, the content of reactive oxygen species and DNA fragmentation in coleoptiles of maize etiolated seedlings of different ages and a possibility of...
ВЛИЯНИЕ ХЛОРИДА КАДМИЯ НА ЖИРНОКИСЛОТНЫЙ СОСТАВ ВЫСШИХ ВОДНЫХ РАСТЕНИЙ РЕКИ АНГАРА
Проведён сравнительный анализ содержания жирных кислот в тканях Myriophyllum spicatum L. и Elodea canadensis Michx. в условиях воздействия 0,05 М раствора хлорида кадмия в течение 24 часов. Показаны изменения в составе ж...
ABA biosynthesis defective mutants reduce some free amino acids accumulation under drought stress in tomato leaves in comparison with Arabidopsis plants tissues
The ability of plants to tolerate drought conditions is crucial for plant survival and crop production worldwide. The present data confirm previous findings reported existence of a strong relation between abscisic acid (...
Effect of NaCl priming duration and concentration on germination behavior of Tunisian safflower
Priming is an effective technique that improves germination of several crop species. That’s why this study was carried in order to evaluate the effect of NaCl seed priming techniques on germination and early growth of sa...