FREE RADICAL SCAVENGING, ANTIOXIDANT POTENTIAL, AND NITRIC OXIDE INHIBITION IN HUMAN THP1 DERIVED MACROPHAGES BY KOKILAKSHAM KASHAYAM
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 8
Abstract
Objective: The aim of this study was to evaluate the antioxidant potential of Kokilaksham kashayam and its effect on the production of nitric oxide (NO) by lipopolysaccharide (LPS) stimulated THP1 derived macrophages. Methods: Kokilaksham kashayam was subjected to fractionation and assessed for antioxidant activity. The effect of fractions on cell viability was determined using 3-(4,5 – dimethylthiazol -2-yl)-2,5-diphenyltetrazolium bromide assay and the fractions were evaluated for their effect on the production of NO by LPS stimulated THP1 derived macrophages. Results: It was found that the fractions of the herbal decoction were able to scavenge a variety of free radicals 2,2-diphenyl-1-picrylhydrazyl, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, NO, and hydroxyl radical. Ferric reducing antioxidant power assay showed the antioxidant capacity and cell culture studies in THP derived macrophages showed that the fractions inhibited the production of NO in LPS-stimulated THP1 derived macrophages. Conclusion: The overall study showed that the proinflammatory role of free radicals in general and specifically NO in chronic inflammatory condition could be managed by the use of Kokilaksham kashayam. The inhibitory effect of Kokilaksham kashayam on NO production and free-radical scavenging activity, in general, proves that the vital phytoconstituents in the herbal decoction are responsible for the antioxidant activity thereby preventing or slowing the process of chronic inflammatory conditions.
Authors and Affiliations
TEENA MERLIN , PRAKASH KUMAR B
Keywords
Related Articles
- EP ID EP636138
- DOI -
- Views 112
- Downloads 0
QUALITY CONTROL EVALUATION OF IN HOUSE PREPARED POLYHERBAL AYURVEDIC FORMULATION SAPTAVIMSATIKA GUGGLU
Introduction: Plants and their constituent are having potential of treating any human suffering in form of disease. Various combination of plant extract is used as a remedy for treating these disorders. However the usage...
EVALUATION OF ANTI-ANXIETY ACTIVITY OF SILICA GEL ENTRAPPED TRADITIONAL MEDICINE
 Objectives: The present study has been designed to compare the anxiolytic property of Makaradhwaja (a well-known traditional Ayurvedic medicine)and gel encapsulated Makaradhwaja in laboratory animals.Methods: Makaradhw...
A RECENT UPDATE: ANTIMICROBIAL AGENTS CONTAINING PYRAZOLE NUCLEUS
Objective: In this review, we report antimicrobial candidates containing pyrazole nucleus integrated with various functionalities.Methods: research results by numerous scientists have been summarized from international j...
MULTIPLE ADVERSE EFFECTS OF BETAMETHASONE USED AS SELF-MEDICATION: A CASE REPORT
 Corticosteroids are commonly prescribed as anti-inflammatory, immunosuppressant and replacement therapy. Adverse effects of corticosteroids arewidely recognized. However, due to easy availability and relief offered, th...
CHEMOPROTECTIVE EFFECT OF EDIBLE GASTROPOD, XANCUS PYRUM AND ITS USEFULNESS IN THE AMELIORATION OF CISPLATIN-INDUCED TOXICITY
  Objective: The main purpose of this study was to evaluate chemoprotective activities of methanolic extracts of an edible gastropod (Xancus pyrum) in cisplatin-induced immunosuppressed mice.Methods: Cisplatin (100 mg/...