Frequency and antibiogram pattern of gram positive cocci in catheter related blood stream infections (CRBSI) in a tertiary care hospital, Tamilnadu

Abstract

Introduction: Blood stream infection associated with catheter is life threatening. Various bacterial agents are responsible for this and Coagulase Negative Staphylococci (CoNS) is now becoming one of the major agent in Catheter Related Blood Stream infection (CRBSI). Objectives: To know the bacterial isolates and the frequency of the CoNS & its antibiotic resistance patterns of in Catheter Related Blood Stream infection (CRBSI). Methods: Catheter tips (5 cm tip) were collected and 5 ml of peripheral blood sample was collected with proper aseptic precautions. Catheter tips were processed using Maki DG et al procedure. Blood was inoculated into the BACTEC blood culture bottle (BacT/ALERT 3D automated blood culture system –BioMerieux). All the gram positive bacteria were further processed for standard biochemical tests. All these isolates were subjected to antibiotic susceptibility testing (Hi-Media discs) by Kirby Bauer disc diffusion test according to CLSI guidelines. Results: Among the total 158 isolates, 55 (34.8%) were gram positive cocci. Coagulase Negative Staphylococci (CoNS), were the predominant isolate in this study. Highest resistance was observed with ampicillin, penicillin, amoxyclav, erythromycin and tetracycline. All the gram positive isolates were 100% sensitive to Vancomycin and Linezolid. Conclusion: Among the total 158 isolates, 55 (34.8%) were gram positive cocci. Coagulase negative staphylococci (CONS) which account 18.98%, followed by 8.86%, Staphylococcus aureus, 5.06% Enterococci and 1.89% were Micrococci. All our isolates were sensitive to Vancomycin and linezolid.

Authors and Affiliations

Rajesh J. G, Senthamarai S, Sivasankari S, Anitha C, Somasunder V, Akila Akila, Kumuthavathi Kumuthavathi

Keywords

Related Articles

Distribution of blood groups among blood donors at a tertiary care hospital in Southern Rajasthan

Introduction: Blood grouping is one of the essential aspects of blood transfusion practice. ABO and Rhesus (Rh) blood groups are the two most important blood group systems among the various types identified so far. Distr...

Histomorphological spectrum of eyelid lesions–A 6 year retrospective study

Background: Eyelid lesions are encountered by all primary care physicians and Ophthalmologists. Histology of eyelid comprises various components and structures that give rise to a wide spectrum of pathologies. The clinic...

Microbial profile of street food from different locations at Tumkur, India

Introduction: The street food provided in ready –to-eat form are prepared and sold by vendors and hawkers in the street and other public places are a major source of foodborne diseases. Aim: The present study was underta...

Analysis of factors affecting the semen pathology in male infertility

Background: Abnormalities in the male are the sole cause of infertility in approximately 20% of infertile couple and important contributing factor in another 20-40% of couple with reproductive failure. Despite our abilit...

Correlation of prostate specific antigen level with histopathological findings in patients with prostatic disease

Background: Carcinoma of prostate is one of the common tumors of old age in men. Although there is great apprehension in these patients when they are associated with even mild increase in the prostate specific antigen le...

Download PDF file
  • EP ID EP263196
  • DOI 10.17511/jopm.2017.i2.04
  • Views 126
  • Downloads 0

How To Cite

Rajesh J. G, Senthamarai S, Sivasankari S, Anitha C, Somasunder V, Akila Akila, Kumuthavathi Kumuthavathi (2017). Frequency and antibiogram pattern of gram positive cocci in catheter related blood stream infections (CRBSI) in a tertiary care hospital, Tamilnadu. Pathology Update: Tropical Journal of Pathology and Microbiology, 3(2), 102-106. https://europub.co.uk/articles/-A-263196