FREQUENCY OF HUMAN LEUCOCYTES ANTIGEN (HLA) B LOCUS IN UMBILICAL CORD BLOOD OF DIFFERENT ETHNIC SUBJECTS IN PORT HARCOURT
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2018, Vol 5, Issue 12
Abstract
The HLA system is known to be the most polymorphic genetic loci in humans. Distribution and frequencies of HLA alleles are highly variable among different human ethnic groups. The aim of the study was to determine the HLA B locus alleles and frequencies in umbilical cord blood in Port Harcourt, Rivers State, Nigeria. Frequencies of the HLA B locus alleles was determined in 90 unrelated umbilical cord blood samples using Sequence Specific Primer-Polymerase Chain Reaction(SSP-PCR) method. The results showed that a total of 25 HLA-B were found in the studied population. Out of which HLA B*53 (32.2%) was the most prevalent followed by B*15(28.8%), B*07(24.4%), B*35 (18.8 %) and B*76(13.3 %). The frequency of HLA B locus alleles among the participating ethnic groups in the study population like the Igbo, Hausa, Yoruba, Ikwerre, Ogoni, Ijaw, Efik and Fulani were also described. The most frequent alleles among the Igbos are HLA B*07(17.6%), HLA B*15(30.4%), HLA B*35(15.2%), HLA B*53(30.4%) and HLA B*76(13.0%). Among the Hausa are HLA B*07(28.6%), HLA B*15(14.3%), HLA B*27(28.6%), HLA B*35(28.6%), HLA B*46(14.3%), HLA B*52(14.3%), HLA B*53(28.8%) and HLA B*57(28.6%). Among the Yorubas are HLA B*07(23.5%), HLA B*15(29.4%), HLA B*35(23.5%), HLA B*53(29.4%) and HLA B*73(11.8%). Among the Ikwerre are HLA B*14, HLA B*15, HLA B*21, HLA B*35, HLA B*41, HLA B*42, HLA B*44, HLA B*53, HLA B*58 and HLA B*78 with allele frequencies of 12.5%, each. The common alleles for Ogoni are HLA B*15, HLA B*53(50.0%), HLA B*76(50.0%), HLA B* 18(25.0%) and HLA B* 58(25.0%). Among the Ijaw are HLA B*07(100%) and HLA A*35(100.0%). HLA B*07(80.0%), HLA B*15(20.0%) and HLA B*18(20.0%), HLA B*35(20.0%) and HLA B*76(40.0%) were found to be common among the Efik. Chi Square test was performed on data set to determine strength of association amongst the ethnic groups in HLA B locus allele frequency. The 2 value was 1579.2, p value 0.0001. The HLA B locus alleles are statistically significantly different across ethnic groups in Nigeria. In conclusion, the result of the study suggests a high variability in HLA B locus in the studied Nigerian population.
Authors and Affiliations
Nwagu Chinyere
Keywords
Related Articles
- EP ID EP646135
- DOI -
- Views 118
- Downloads 0
UNDERSTANDING THE MODE OF ACTION AND EFFICACY OF TAKRADHARA
Dhara is one of the popular therapy in Panchkarma. Dhara is a procedure in which medicated oil, milk or buttermilk is poured in a continuous stream on head especially on forehead and when ‘Takra’ is poured on forehead an...
LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY (LC-MS) - AN UPDATE
Mass Spectrometry (MS) is an important analytical tool for molecular research and used around the globe to identify, quantify and characterize biomolecules like proteins from any number of biological conditions or sample...
EFFECTS OF SHELL ON THE NUTRITIONAL VALUE OF ROASTED GROUNDNUTS SEEDS
Groundnut (Arachis hypogaea) seeds (raw, roasted with shell and roasted without shell) were analyzed for proximate composition and oil extracted from the samples was subjected to physicochemical analysis. The results obt...
A LUMBAR HERNIA: A RARE CONGENITAL ANOMALY
Lumbar hernias are very rare, less than 300 cases have been reported over the past 300 years.[1] Lumbar hernias manifest through two possible defects in the posterior abdominal wall either in superior (Grynfeltt) or infe...
EVALUATION OF MEDICATION ERRORS AND THEIR REDUCTION IN PEDIATRIC ANESTHESIA SETTING
Aim of study: The study aimed to characterize the frequency, type and outcome of anesthetic medication errors among children during 8-year period. Patients: Patients of this study were children selected from Department o...