Frequency of Human Platelet Antigens (HPA-2/3/5) Polymorphism in Iranians Evaluated by RFLP-PCR
Journal Title: Iranian Journal of Blood and Cancer - Year 2010, Vol 2, Issue 4
Abstract
Background: Knowing the human platelet antigens (HPA) and genes frequency in different populations is important not only for population studies but also for clinical transfusion practice. HPA genes frequency in Iran is not evaluated, so we investigated the HPA-2, HPA-3, and HPA-5 frequency and their polymorphism. Materials and Methods: DNA from 120 Iranians (99 Iranian blood donors and 21 patients with platelet refractoriness) was isolated from peripheral blood mononuclear leucocytes and tested by restricted fragment length polymorphism analysis (RFLP). Results: The frequency of HPA phenotypes was determined as follows: (in donors) HPA-2a/2a:75.4%, HPA-2a/2b:24.8%, HPA-3a/3a:8.1%, HPA-3a/3b:70.7%, HPA-3b/3b:21.2%, HPA-5a/5a:99 % and HPA-5a/5b:1% (in patients) HPA-2a/2a:66.7%, HPA-2a/2b:33.3%, HPA-3a/3a:23.8%, HPA-3a/3b: 47.6%, HPA-3b/3b:28.6%, HPA-5a/5a:90.5%, and HPA-5a/5b: 9.5%. Frequency of genotypes evaluated by RFLP were as below: HPA-2a=0.87, HPA-2b=0.13, HPA-3a =0.28, HPA-3b=0.72, HPA-5a=0.99, and HPA-5b=0.01 in both donors and patients. Conclusion: HPA gene frequencies observed in Iranians were in the range obtained in previous studies but not exactly equal to other populations. It might be due to their relations with other populations. There was not any significant difference between two groups in this study.
Authors and Affiliations
Shaban Alizadeh, Mojgan Shaiegan, Shahram Samiei, Zahra Ataee, Tahereh Madani, Jahangir Ahmadi, Azita Azarkeivan, Leila Kasraian
Epidemiology of Patients with Cancer in Seventh Tir General Hospital from 1992 to 2005
Background: Population based research contributes to our understanding of cancer at many levels. For health program planning and policy making it is important to have information about the incidence of the common cancers...
Hypoxia Preconditioning Promotes Survival And Clonogenic Capacity Of Human Umbilical Cord Blood Mesenchymal Stem Cells
Background: In recent decade, human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) provide enormous potential for appropriate cell therapy, but they have limited growth potential and cease to proliferate...
Hemoglobin E/β⁰ Thalassemia in south west Iran - A case series
Background: Beta thalassemia gene mutations are among common mutations in southwest Iran. However, Hemoglobin E (Hb E) and Hb E/β⁰ thalassemia account for a small number of hemoglobinopathies in Iran. This is the first s...
Role of Ghrelin in Cancer
Cancer is one of the most fatal diseases in human beings which annually leads to death of 30000 individuals in Iran. Prevention, diagnosis and treatment of cancer is one of the major scientific challenges all around the...
A Rare Presentation of Arterial Thrombosis after Venous Thrombosis in a Patient with Gastric Cancer
Background: Venous thromboembolism, which comprises deep vein thrombosis and pulmonary embolism and is a significant cause of morbidity and mortality among patients with cancer. The aim of the present study was to report...