Frequency of Torque Teno Mini Virus in Hepatitis B and C Patients and Healthy Blood Donors in Isfahan, Iran
Journal Title: Iranian Journal of Blood and Cancer - Year 2014, Vol 6, Issue 3
Abstract
Background: Recently, some new viruses have been identified for their association with hepatitis which Torque Teno Mini Virus being among them. The aim of this study was to determine the frequency of Torque Teno Mini Virus in healthy individuals and hepatitis B and C patients in Isfahan, Iran. Materials and Methods: One hundred serum samples of healthy individuals from Isfahan Blood Transfusion Organization were collected. A total of 25 human serum samples from hepatitis B and 25 samples from hepatitis C infected patients were also collected from Mahdieh diagnostic laboratory in Isfahan, Iran. Viral DNA was extracted and Torque Teno Mini Virus DNA was detected using a nested PCR with primer sets designed for a conserved region of the Torque Teno Mini Virus genome. PCR and Reverse transcriptase PCR were used for detection of HBV and HCV respectively. Results: Torque Teno Mini Virus -DNA was detected in 17% of healthy individuals. It also was detected in 20% and 48% of serum samples from hepatitis B and C infected individuals, respectively. The frequency of Torque Teno Mini Virus was significantly higher in hepatitis C patients versus healthy individuals (P < 0.05). Also, the frequency of TTMV in hepatitis C patients was significantly higher than hepatitis B patients (P < 0.05). Conclusions: The difference in Torque Teno Mini Virus frequency between the hepatitis C and healthy group was significant (P< 0.05). The etiology of the higher infection rate in hepatitis C individuals needs to be determined. Keywords: Torque Teno Mini Virus, Hepatitis B, Hepatitis C, PCR.
Authors and Affiliations
Alireza Ghazimorad, Majid Bouzari, Mohammad Taghi Kardi
Keywords
Related Articles
- EP ID EP364641
- DOI -
- Views 70
- Downloads 0
Expression Analysis of Foxo3a Gene in Pediatric Acute Lymphoblastic Leukemia in Southern Iranian Population
Background: Acute lymphoblastic leukemia (ALL), the most common childhood cancer with a peak incidence in children from 2-5 years old, might be associated with poor prognosis and resistance to therapy in specific cytogen...
The Correlation between Infants’ Birth Weight and Apgar Score and Maternal Hemoglobin Concentration
Background: Various studies have come to contradictory results regarding the relationship between maternal hemoglobin (Hb) level and adverse pregnancy outcomes. The present study aimed to investigate the birth outcomes a...
Detection of Factor VIII Inhibitors in Hemophilia A Patients
Background: Factor VIII administration to hemophilia A patients results in an immune response (inhibitor formation) which significantly complicates the therapy. The present study was performed to determine the prevalence...
New Understandings of Platelet Function
This article has no abstract.
Cognitive, Emotional, and Behavioral Problems of Children with Hemophilia
Background: Children with hemophilia are prone to a variety of psychological problems due to some limitations associated with the disease. We aimed to compare the cognitive, emotional, and behavioral problems of children...