Fribrinolytic activity and gas production by Pleurotus ostreatus-IE8 and Fomes fomentarius - EUM1 in bagasse cane
Journal Title: Revista MVZ Cordoba - Year 2015, Vol 20, Issue 3
Abstract
Objective. To characterize the fibrolytic enzymatic activity of Pleurotus ostreatus-IE8 and Fomes fomentarius-EUM1 in sugarcane bagasse (BCA); to evaluation of the kinetics of in vitro production of BCA treated by solid fermentation (FS), crude enzyme extract (ECE) of P. ostreatus-IE8 and Fibrozyme®. Materials and methods. In fungi measured radial growth rate ( Vcr ) and biomass production in two culture media (with or without nitrogen source); activity of xylanases, cellulases and FS on BCA at 0, 7 and 15 d. The chemical analysis and kinetic analysis of in vitro gas production in 4 treatments (ECE adding enzymes obtained from the direct addition FS or FS ), witness (Fibrozyme®) and a control without addition and analyzed by a was completely randomized design. Results. Xylanases (7 d ) showed 6.32 and 5.50 UI g-1 initial substrate dry weight (SSi) for fungi P. ostreatus-IE8 and F. fomentarius-EUM1 , respectively ; P. ostreatus-IE8 scored higher activity of laccases (10.65 g -1 UI SSi) and F. fomentarius-EUM1 (1.90 UI g-1 SSi) cellulases. The ECE of P. ostreatus-IE8 and commercial enzyme did not differences (p>0.05). In the chemical composition or the gas production kinetics. The 4 treatments evaluated decreased values of the variables measured in the kinetics of gas production compared to the control (p≤0.05). Conclusions. The ECE of P. ostreatus-IE8 was similar to commercial enzyme degradation in vitro, so it is feasible to use pre-digest high fiber products.
Authors and Affiliations
Paulino Sánchez-Santillán, Ph. D| Colegio de Postgraduados, Campus Montecillo. Carretera México-Texcoco, Km 36.5, Montecillo, Estado de México, México, C.P. 56230.Universidad Autónoma de Guerrero, Unidad Académica de Medicina Veterinaria y Zootecnia N°2, Carretera Acapulco Pinotepa Nacional Kilómetro 197, Cuajinicuilapa, Guerrero, México, C.P. 41940, Marcos Meneses-Mayo, Ph. D *| Universidad Anáhuac México-Norte, Facultad de Ciencias de la Salud (Nutrición), Av. Universidad Anáhuac No 46, Colonia Lomas Anáhuac Huixquilucan, Estado de México, México, C.P. 52786.Correspondence: marcos.meneses@anahuac.mx, Luis Miranda-Romero, Ph. D| Universidad Autónoma Chapingo, Departamento de Zootecnia. Carretera México-Texcoco, Km 38.5, Chapingo, Estado de México, México, C.P. 56230., Eduardo Santellano-Estrada, Ph. D| Universidad Autónoma de Chihuahua, Facultad de Zootecnia. Periférico Francisco R. Aldama, km 1, Chihuahua, Chihuahua, C.P. 31543., Baldomero Alarcón-Zúñiga, Ph. D| Universidad Autónoma Chapingo, Departamento de Zootecnia. Carretera México-Texcoco, Km 38.5, Chapingo, Estado de México, México, C.P. 56230.
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