Frist Detection of The blaOXA-23 Genes in Clinical Isolates of Acinetobacterbaumanniiin Babylon Hospitals-Iraq
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 4
Abstract
Acinetobacter spp. have risen as important pathogens in medicinal services related contaminations. Carbapenems are vital antimicrobial agents for treating diseases due to multidrug resistant Acinetobacter spp. Resistance to these drugs in the genus, may beconferred by different mechanisms particularly production of class D carbapenemases. OXA-23-like family has been brought up as one of the dominating carbapenemases among Acinetobacter. The present study aimed to investigate the occurrence of OXA-23-like carbapenemases among Acinetobacterisolatesrecovered from patients of hospitals in Babylon, Iraq. Antimicrobial susceptibility profiles were determined by disk-diffusion method.Imipenem resistant isolates were submitted to Modified Hodge Test and Double Disk Synergy Test to screen for carbapenemase generation, and later to polymerase chain reaction (PCR) to explore the presence of blaOXA-23 genes . One (10%) of isolates was observed to be imipenem and meropenem resistant (MIC > 512 μg/ml). 6(60%) gave positive results with the imipenem-EDTA disk and Modified Hodge test ; 4 isolates (40%) as indicated by PCR results, carried the blaOXA-23 genes. OXA-23-like enzymes might be a critical component of carbapenem resistance among isolates present in the hospitals studied.
Authors and Affiliations
Raad A. AL- Harmoosh, Eman M. Jarallah**, Anmar M. AL- Shamari*, Hussein M. AL-khafaji, Sura I. Jabuk, Essam M. AL-Khaweledy
Keywords
Related Articles
- EP ID EP385531
- DOI 10.9790/3008-1104024448
- Views 81
- Downloads 0
Quantized ATP Production
Although ATP production is very important for mitochondria, little is known about the actual rate of production and the value of “unit of currency” for mitochondrial energy transactions. “Unit of currency” for all transa...
Prevalence and management practices of malaria in a rural community of Ebonyi state, southeast Nigeria
A prevalence survey of malaria was carried out in Onicha-Igbeze, a rural community in Onicha Local Government Area of Ebonyi state, southeast Nigeria. Thick blood films of 100 individuals in the community were used to de...
Role Of Bioregulator On Proline, Proteins And Soluble Sugar Accumulation In Organs Of Okra Under Salinity Stress
Okra is an important vegetable grown in tropical and sub-tropical regions. Here, we study its response to salinity stress. Seeds of 47-4 and LD88 genotypes were pre-soaked with indole acetic acid (0.4, 0.5 and 0.6mM) and...
Effect of Gum Arabic on Liver Function And Antioxidant Enzymes of Sprague-Dawley Rats
The aim of this study was to investigate the effect of twelve weeks Gum Arabic dose on the liver functions and some antioxidant enzymes activity of 10 Sprague-Dawley rats. Methods: 10 normal Sprague-Dawley rats were fed...
Extraction and purification of ßeta-galactosidase from a Local Almond seeds(Prunusamygdalus)
ßeta-galactosidaseenzyme is greatly dispersed in plant tissues and it’s known to be implicated in hydrolyzing terminal non reducingß.D.Galactosyl residues from polysaccharides . This study involved the selection of the b...