Fructose-1,6-diphosphate and vitamin C combination increases seizure threshold in chemical kindling models
Journal Title: National Journal of Physiology, Pharmacy and Pharmacology - Year 2017, Vol 7, Issue 3
Abstract
Background: Fructose-1,6-diphosphate (FDP), through its activity in glucose metabolism, has anticonvulsant efficacy whereas vitamin C acts as neuromodulator. Aims and Objectives: To assess the antiepileptic effects of FDP, vitamin C and their combination in intravenous pentylenetetrazole (PTZ) and intracranial carbachol kindling model. Materials and Methods: PTZ kindling was induced by intravenous infusion of PTZ (10 mg/ml; infusion rate: 1 ml/min) to rats. For carbachol kindling, intracranial administration of carbachol (1 µl; 5 µg/ml) was done at well-spaced intervals by introducing maximum three injections a day, and maximum nine administrations until stage 5 epileptic convulsions were produced. The treated group animals received FDP, vitamin C or both, 28 days before the kindling. During kindling, time latency to reach a seizure stage, electroencephalogram (EEG) recording, and frequency of stimulation were recorded. Brain homogenate parameters included measurement of levels of neurotransmitters, glutathione (GSH), and malondialdehyde (MDA). One-way ANOVA was used for determination of a significant difference between the groups. Results: Slowing of seizure progression, increase in seizure threshold and required number of stimuli to reach to stage 5 convulsion was highest with FDP and Vitamin C combination followed by FDP and Vitamin C alone treatment. EEG data during the PTZ infusion indicated ictal epileptiform in disease control group and not in treatment groups. The test drugs either alone or in combination did not alter glutamate and aspartate levels in brain but increased the γ-aminobutyric acid and GSH whereas reduced MDA levels. Conclusion: It can be concluded that both FDP and Vitamin C has beneficial effects in chemical induced kindling and their combination shows synergistic effects.
Authors and Affiliations
Bela Paras Samariya, Gaurang B Shah
Keywords
Related Articles
- EP ID EP289208
- DOI 10.5455/njppp.2017.7.0927330092016
- Views 96
- Downloads 0
Description of physical fitness, blood glucose, and cholesterol levels of the middle age operational workers in transportation company
Background: Physical fitness (PF) is a key to maintain company productivity. The transportation company has complicated jobs and high physical demand. Aging, obesity, and sedentary lifestyle can decrease PF and work prod...
Cutaneous adverse drug reactions from a teaching hospital in Bengaluru: An observational study to determine the spectrum and outcome
Background: Cutaneous adverse drug reactions (CADRs) are frequent manifestations of drug reactions that can lead to discontinuation of treatment, impaired quality of life and increased economic burden. Knowledge of drugs...
Assessment of drug use pattern using World Health Organization prescribing indicators in a tertiary care hospital in Mangalore: A cross-sectional study
Background: Irrational prescription being a global problem leading to ineffective, unsafe treatment. Aiming to measure the performance of a health-care provider in key dimension related to appropriate use of drugs, the W...
Does visceral fat affect aerobic fitness in Indian adolescents of 18-19 years’ age group?
Background: Cardiovascular endurance tests are considered as one of the best methods for assessment of the individual’s health. Reduced aerobic fitness is seen in overweight and obese. Aerobic fitness is also reduced in...
Eugenol efficacy in preventing nicotine-induced seizures in mice
Background: Seizure is the most common neurological disorder after stroke. In fact, the seizure is a chronic neurological disorder affected 1-2% of the global population. Given the side effects and toxicity of synthetic...