Fungal Infection Enhances Anthocyanin Production in Blue Flowers of Clitoria ternatea
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 7
Abstract
Aims: A white fungus which commonly infects blue petals of Clitoria ternatea L. (CT) was identified and total anthocyanin content and antioxidant activity of CT extracts from infected and uninfected CT were compared. Study Design: Experimental research. Place and Duration of Study: Samples of infected and non-infected CT were collected from Muang, Khon Kaen and chemical analysis was undertaken at Khon Kaen University between July and December 2012. Fungus was identified at Department of Microbiology in April 2013. Methodology: Samples of dried CT, with and without white fungus infection, were collected. The CT-infected fungus was isolated for microscopic characterization and DNA isolated for amplifying of its ITS rDNA, and analyzing their sequences with neighbor joining phylogenetic tree. Total anthocyanin content by pH differential method and antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) assay were used to compare infected and non-infected CT extracts in comparison to trolox. Both extracts were analyzed by HPLC in comparison to cyanidin. Results: Morphology and DNA of the infected fungus was characterized and confirmed to be Penicillium citrinum KKU-Rx1. In comparison to aqueous extracts of non-infected CT, infected CT gave about 1.5 times higher total anthocyanin content (P=.002) and cyanidin (P = .009). Trolox equivalent antioxidant capacity of infected-CT extracts was 1, while that of non-infected CT extracts was 0.7 (P<.001). Conclusion: Penicillium citrinum was identified from white fungus infection on deep blue flowers of Clitoria ternatea and shown to enhance the anthocyanin content and antioxidant activity of the CT extracts.
Authors and Affiliations
Aroonsri Priprem, Sophon Boonlue, Natanong Yodsing, Bhattaranitch Khampaenjiraroch
Keywords
Related Articles
- EP ID EP237688
- DOI 10.9734/BBJ/2014/10370
- Views 101
- Downloads 0
Genotyping and Nucleotide Sequences of Growth Hormone Releasing Hormone and Its Receptor Genes in Egyptian Buffalo
Aim: The hypothalamic hormone, growth hormone-releasing hormone, is the principal stimulator of pituitary growth hormone (GH) synthesis and secretion. GHRH and its receptor (GHRHR) provide important functions in the regu...
Recovery of Biomass Energy on the Farm: Identification of Cellulolytic Bacteria in Agricultural Residues for On-Site Bioethanol Production
Farms are significant sources of unused biomass, conversion of which into energy would contribute to decreasing the environmental footprint associated with farming activities. A promising alternative for energy conversio...
Production and Partial Characterization of Pectinase from Snail (Archachatina marginata) Gut Isolates
Aim: To identify isolates for industrial production of pectinase. Methodology: The isolates were screened for pectinolytic activity using pectin as substrate. Enzyme activity was expressed as mg of glucose equivalent rel...
Fungal Infection Enhances Anthocyanin Production in Blue Flowers of Clitoria ternatea
Aims: A white fungus which commonly infects blue petals of Clitoria ternatea L. (CT) was identified and total anthocyanin content and antioxidant activity of CT extracts from infected and uninfected CT were compared. St...
Antitheilerial Herbal Medicine: A Review
The aim of this study is to overview some of the medicinal plants tested for the treatment of theileriosis. Treatment of theileriosis with standard drugs such as parvaquone and buparvaquone is very expensive, and these d...