Gamma and UV radiation induced mutagenesis in Trichoderma reesei to enhance cellulases enzyme activity
Journal Title: International Journal of Farming and Allied Sciences - Year 2014, Vol 3, Issue 5
Abstract
The need for renewable energy resources has focused attention on the renewable polysaccharide cellulose, which can be enzymatically hydrolyzed to yield sugars. The filamentous fungus Trichoderma reesei is an important host organism for industrial enzyme production producing large amounts of hydrolytic enzymes. The aim of the present study was to improve the cellulase production of T. reesei through a successive mutagenesis step. Trichoderma reesei (PTCC 5142) a cellulolytic microorganism, was subjected to mutagenesis using gama and UV radiation individually as mutagens. Successive mutants showed rapid growth on plates containing MYG medium, clear hydrolysis zone on mandels medium and enhanced cellulase production. The mutant strains of T. reesei were isolated and selected by mandel screening method. Results showed some mutant strains has had higher cellulases activities in comper with parent strain. A mutant strain of Trichoderma reesei (PTCC 5142) with maximum production potential of cellulase was finally selected. The developed mutant from gama mutagenesis way is the best mutant for Ccase, CMCase, Avicellase and Fpase activities. This new method could be applied to obtain potent fungal mutants for more enzymes production.
Authors and Affiliations
Samira Shahbazi, Khodayar Ispareh, Mahsa Karimi, Hamed Askari and Mohammad ali Ebrahimi
Keywords
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