GC-MS ANALYSIS OF BIOACTIVE COMPOUNDS AND ANTIMICROBIAL ACTIVITY OF CRYPTOCOCCUS RAJASTHANENSIS KY627764 ISOLATED FROM BOMBYX MORI GUT MICROFLORA.
Journal Title: International Journal of Advanced Research (IJAR) - Year 2018, Vol 6, Issue 3
Abstract
The insect gut microflora acts as a large reservoir of bioactive natural products as a diversity of resident microflora is found to be symbiotically associated with the insect. The present study reports the bioactive chemical compounds and the antimicrobial nature of the yeast isolated from the insect Bombyx mori gut. The yeast is identified as Cryptococcus rajasthanensis by Molecular characterization. The Fourier transform infrared spectroscopy (FT-IR) studies of the chloroform and ethyl acetate crude yeast extracts indicated a number of functional groups like alcoholic, phenolic, ester, aldehydic, etc. that accounts for the bioactive nature of the extracts. The Gas chromatography-Mass spectroscopy (GC-MS) analysis of the crude extracts revealed a large number of bioactive compounds of high and low molecular weight that are considered biologically active. Some of the biologically active molecules like phenol 2,4-bis(1,1-dimethylethyl), 1,2-benzenedicarboxylic acid dibutyl ester, celidoniol deoxy, nonadecane, tetratetracontane, 2-methyloctacosane and pentadecane bearing antimicrobial property were detected in both chloroform and ethyl acetate extracts. Furthermore, the crude extracts were subjected to antimicrobial assay by agar well diffusion method and are found to be antimicrobial against four pathogenic bacteria Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa and two fungi Candida albicans and Aspergillus flavus. The chloroform extract showed maximum inhibition for E. coli and minimum for A. flavus.
Authors and Affiliations
Delicia Avilla Barretto, Shyam Kumar Vootla.
Keywords
Related Articles
- EP ID EP281039
- DOI 10.21474/IJAR01/6700
- Views 55
- Downloads 0
MESENTERIC CASTLEMAN\'S DISEASE ACCIDENTAL DISCOVERY OF A CASE OF MESENTERIC CASTLEMAN\'S DISEASE IN THE FORM OF AN ABDOMINAL MASS.
Castelman disease is known as benign angiofollicular node hyperplasia disorder; mostly described in mediastinum. The mesenteric localisation is exceedingly rare, that can be ignored in diagnosis front of an abdominal mas...
A STUDY TO COMPARE EFFICIENCY OF HBA1C, FASTING & POST-PRANDIAL BLOOD GLUCOSE LEVELS, IN THE DIAGNOSIS OF TYPE-2 DIABETES MELLITUS AND ITS PROGNOSTIC OUTCOME.
Background: HbA1C a marker of chronic hyperglycemia, is associated with diabetes and its complications and has been recommended as a diagnostic test. It is an indicator of average blood glucose concentration over the per...
EVALUATION OF SODIUM CHLORIDE STRESS TOLERANCE IN FINGER MILLET (ELEUSINE CORACANA L.) CULTIVARS BY OBSERVING MORPHOLOGICAL CHARACTERS.
Saline soils are unproductive. Plant growth and yield are highly effected by salinity. This may overcome by cultivating salt tolerant varieties. The aim of this study is to figure out, salt tolerant and salt sensitive cu...
THE RELATIONSHIP BETWEEN PERSONALITY TRAITS, AND SELF-EFFICACY ON THE LEVEL OF STUDENTS TEST ANXIETY.
This research aimed at studying the relationship between personality traits, and self-efficacy in the level of student with test anxiety. One hundred ten part-time students (male and female) from the second year and four...
THE ERA OF CLOUD COMPUTING: A RECENT SURVEY.
Cloud computing an emerging technology provides various services to the users like infrastructure, hardware, software, storage etc. For working cloud in data security, it is necessary that cloud computing network should...