GC-MS analysis of yellow pigmented Macrococcus equipercicus isolated from alfalfa rhizosphere soil fields of Coimbatore
Journal Title: Journal of Applied and Natural Science - Year 2019, Vol 11, Issue 3
Abstract
The rhizosphere of plant possesses important microflora, which secretes wide chemical compounds including secondary metabolites necessary for plant growth and development. The microbial flora of alfalfa plant rhizosphere soil region was explored for functional activity and we found upto ten different pigmented colonies. Due to good functional diversity, this yellow pigmented colony was taken for further studies. Thus, the culture was molecularly characterized and identified for potent bioactive components responsible for antimicrobial activity. The selected culture mass was cultured and secondary metabolites were produced and extracted using ethyl acetate and subjected to GC-MS analysis. The antimicrobial study revealed selective activity against Streptococcus pneumonia, and Proteus sp with zone of inhibition to be 18 and 20 mm respectively. Molecular identification of the isolate by 16S rRNA sequencing showed the isolate as Macrococcus equipercicus with 100 % similarity. Based on GC-MS analysis report 25bioactive compounds were identified and 13-docosenamide, hexadecanoic acid esters and quercetin were found in ethyl acetate extract. Conclusion: Thus the yellow pigmented gram positive cocci M.equipercicus isolated from Medicago sativa possessed wide antibacterial activity due to presence of quercetin. Through the studies, we were able to identify potent antibacterial compound producing bacteria from M. sativa plant rhizosphere soil.
Authors and Affiliations
Z. Aiysha Thasneem, K. Aravindh, M. Jeba Malar Fencia, C. Nitheesh Kumar, T. Pavithra, S. Surendran, R. Mahesh , S. Ramalakshmi
Keywords
Related Articles
- EP ID EP632216
- DOI 10.31018/jans.v11i3.2139
- Views 66
- Downloads 0
Priority queueing model with balking and reneging
This investigation deals with two levels, single server preemptive priority queueing model with discouragement behaviour (balking and reneging) of customers. Arrivals to each level are assumed to follow a Poisson process...
Effect of plant growth regulators on growth, yield and fruit quality of strawberry (Fragaria x ananassa Duch.) under protected conditions
A field trial was conducted during 2015 and 2016, to study the effect ofgrowth regulators on growth, flowering, yield and fruit quality of strawberry cv. Chandler. The experiment was laid out in completely randomized des...
Population dynamics of potential bioagents of mustard aphid, Lipaphiserysimi(Kaltenbach) on different cultivars of rapeseed-mustard
Field experiment was conducted to study the population dynamics of potential bioagents of mustard aphids on 9 different cultivars which included: Brassica alba cv. PSB-I, Eruca sativa Mill cv. T-27, Brassica campestriscv...
Fungicidal management of Alternaria alternata (Fr.) Keissler causing blight of gerbera (Gerbera jamesonii H. Bolus ex J.D. Hook)
Alternaria, the fungal pathogen has wide host range generally attacks the aerial parts of plants causing leaf spots and blights. Gerbera is a genus of ornamental flower plants. Gerbera plants are infected by many disease...
Phytotoxic impact of Parthenium hysterophorus L. on Macrotyloma uniflorum a pulse crop in a dry tropical environment, Bihar, India
An experiment was conducted to evaluate the effect of aqueous extract of leaf, stem and root of Parthenium hysterophorus on the rate of seed germination (%) and seedling growth (cm) in Macrotyloma uniflorum. The differen...