GC/MS analysis and In-vitro Antioxidant activity of methanol extract of Ulothrix flacca and its main constituent Dimethyl Sulfone.

Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 1

Abstract

The determination of phytochemical constituents, total phenol, flavonoid contents and antioxidant assays of methanol extract of Ulothrix flacca and its main constituent dimethyl sulfone was studied. The mass spectra of the compounds were matched with the NIST library. The GC-MS analysis of methanol extracts of Ulothrix flacca showed sixteen peaks. Of all the sixteen chemical compounds revealed from the GC-MS analysis of Ulothrix flacca, Dimethyl Sulfone (C2H6O2S) (RT-8.9), 4-Bromobenzoic Acid, 2-Chlorophenyl Ester (C13H8BrClO2) (RT-12.642), Tetradecanoic Acid, 10,13-Dimethyl-, Methyl Ester (C17H34O2) (RT-18.669) are the three major components. The methanol extracts of Ulothrix flacca possess phenolic and flavonoid content of (5.74 ± 0.45mg Gallic acid equivalent (GAE)/g Wt, and 12.58 ± 1.52mg quercetin eq/g wt) respectively. Antioxidant activity was determined using 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical, for evaluating free radicle scavenging activity, ABTS radical cation scavenging activity, Ferric reducing antioxidant power (FRAP) assay, Phosphomolybdenum assay and Metal chelating activity using BHT, Rutin and Quercetin. The highest radicle scavenging activity was shown by dimethyl sulfone (15.156mg/ml), which is higher than the BHT and Rutin. In vitro antioxidant activity of methanolic extract of Ulothrix flacca and Dimethyl sulfone showed an increase with increasing concentration indicating positive association with the total phenolic and flavonoid contents of the extract, which could be considered for future applications in medicine, dietary supplements ,cosmetics or food industries.

Authors and Affiliations

B. Vasudevarao . , D. J. Sravanthi2

Keywords

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  • EP ID EP387263
  • DOI 10.9790/3008-12010193104
  • Views 88
  • Downloads 0

How To Cite

B. Vasudevarao . , D. J. Sravanthi2 (2017). GC/MS analysis and In-vitro Antioxidant activity of methanol extract of Ulothrix flacca and its main constituent Dimethyl Sulfone.. IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS), 12(1), 93-104. https://europub.co.uk/articles/-A-387263