GC/MS analysis and In-vitro Antioxidant activity of methanol extract of Ulothrix flacca and its main constituent Dimethyl Sulfone.
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 1
Abstract
The determination of phytochemical constituents, total phenol, flavonoid contents and antioxidant assays of methanol extract of Ulothrix flacca and its main constituent dimethyl sulfone was studied. The mass spectra of the compounds were matched with the NIST library. The GC-MS analysis of methanol extracts of Ulothrix flacca showed sixteen peaks. Of all the sixteen chemical compounds revealed from the GC-MS analysis of Ulothrix flacca, Dimethyl Sulfone (C2H6O2S) (RT-8.9), 4-Bromobenzoic Acid, 2-Chlorophenyl Ester (C13H8BrClO2) (RT-12.642), Tetradecanoic Acid, 10,13-Dimethyl-, Methyl Ester (C17H34O2) (RT-18.669) are the three major components. The methanol extracts of Ulothrix flacca possess phenolic and flavonoid content of (5.74 ± 0.45mg Gallic acid equivalent (GAE)/g Wt, and 12.58 ± 1.52mg quercetin eq/g wt) respectively. Antioxidant activity was determined using 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical, for evaluating free radicle scavenging activity, ABTS radical cation scavenging activity, Ferric reducing antioxidant power (FRAP) assay, Phosphomolybdenum assay and Metal chelating activity using BHT, Rutin and Quercetin. The highest radicle scavenging activity was shown by dimethyl sulfone (15.156mg/ml), which is higher than the BHT and Rutin. In vitro antioxidant activity of methanolic extract of Ulothrix flacca and Dimethyl sulfone showed an increase with increasing concentration indicating positive association with the total phenolic and flavonoid contents of the extract, which could be considered for future applications in medicine, dietary supplements ,cosmetics or food industries.
Authors and Affiliations
B. Vasudevarao . , D. J. Sravanthi2
Keywords
Related Articles
- EP ID EP387263
- DOI 10.9790/3008-12010193104
- Views 64
- Downloads 0
Role of Cinnamon Extract in the Protection against Amoxicillin/Clavulanate-Induced Liver Damage in Rats
Amoxicillin/clavulanate (AC), which is effectively used in the treatment of several number of bacterial infections, may cause hepatotoxicity. Cinnamon extract contains natural products which showed antioxidant, anti-infl...
Phosphatidylinositol 4-Kinase Enzymes: Functional Roles and Potential for Drug Target
The two types of phosphatidylinositol 4-kinases (PI-4Ks) synthesize phosphatidylinositol 4-phosphate (PI-4P), a member of the phosphoinositide family. Phosphoinositides (PIPs) are synthesized from phosphatidylinositol (P...
Genotyping of Human papilloma virus DNA isolated from cervical tissue biopsies of Iraqi women with different cervical abnormalities
Human papillomaviruses(HPV) are the etiological agents of cervical and other ano-genital malignancies. More than 200 genotypes of HPV have been identified, of these, genotypes 16,18,31,33,45,51,52,53,58 have been classif...
Bioactivity-Guided fractionations for Anti-Cancer Property of Leaves of Justicia beddomei
Aim: The primary objective of the study was to evaluate the possible effects of ethanolic, chloroform extracts and isolated constituents of Justicia beddomei leaves (JB) on against Ehrlich’s ascites carcinoma (EAC) induc...
Effect of additives on Pleurotus ostreatus Growth on Agar medium
Oyster mushroom (Pleurotus ostreatus) is one of the most widely grown mushrooms worldwide. This type of fungus have been grown for centuries in green houses to produce mycelium and fruiting bodies and marketed as highly...