GCMS Analysis of Leaf and Salt Stress Callus of Eggplant (Solanum melongena L.)
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 14, Issue 6
Abstract
In the present study, the leaf explant cultured on a MS medium supplemented with BAP (4.44 μM) + NAA (0.98 μM) had the ability to induce more amount of green and friable callus. The 40 days old best grown callus was sub cultured on the combination of various concentrations of BAP (2.22, 4.44, 6.66, 8.88 and 11.1μM) along with same concentrations of NAA (0.98 μM) with different concentrations of NaCl (20, 40, 60, 80 and 100 mM). The highest percentage of callus was observed on MS + BAP (4.44 μM) + NAA (0.98 μM) + NaCl 20 mM with the percentage of 62.33±1.35. The shoot induction was observed on the same media composition with 95% response. Preliminary phytochemical analysis of ethanol and methanol extracts of salt callus showed the presence of alkaloids, saponins, steroids, tannins/ phenolics, flavonoids, glycosides and reducing sugar. GCMS analysis revealed the presence of 27 compounds in the ethanolic extract of S. melongena. Among that there are seven major peaks which indicating the presence of seven major phytochemical constituents. From the twenty seven compounds identified, the most prevailing compounds were, Tetracontane (12.64%), Lupeol (12.44%), N-Tetratetracontane (11.60%), Tetrapentacontane (11.05%) Dotriacontane (10.90%), n-Hexatriacontane (9.18%) and Eicosane (5.26%). Twenty five compounds were identified through GCMS analysis of methanolic salt callus extracts of S. melongena. The most prevailing compounds were 1-Heptacosanol (54.39%), Ergosta-5, 7, 22-trien-3-ol (8.37%), Tetracosane (5.26%), Tetratetracontane (4.49%) and Beta Carotene (4.25%).
Authors and Affiliations
A. Vanitha, K. Kalimuthu, V. Chinnadurai, Y. Sharmila Juliet, R. Prabakaran
Keywords
Related Articles
- EP ID EP342357
- DOI 10.9734/BJPR/2016/30425
- Views 78
- Downloads 0
Advanced Central Effects of Yohimbine on the Cognitive Function, Psychomotor Performance Task and Working Memory: A Randomized Controlled Clinical Trial Study
Systemic administration of yohimbine lead to powerful anxiety-promoting effects and facilitated long-term performances, due to α2- adrenergic receptor action , while its effect on the extinction of reward-related mem...
In Vivo Assay of Analgesic Activity of Methanolic and Petroleum Ether Extracts of Manilkara zapota leaves
Aims: The main objective of this work was to observe the analgesic activity of Manilkara zapota (leaves) on mice. Study Design: The Present study was designed to observe pharmacological activities of the crude extracts...
Rationality and Ethics of Promotional Drug Literature in Medical Journals
Introduction: Currently irrational prescribing is a major concern to community physicians. The promotional drug literature of pharmaceutical companies in medical journal is an important mode to get the information about...
Anti-inflammatory Activity of Cucumis sativus L.
Aims: To evaluate the anti-inflammatory activity and acute toxicity of Cucumis sativus L. Study Design: Animal model assays of anti-inflammatory. Place and Duration of Study: Department of Biochemistry, University of Ni...
Hepatoprotective Activities and Bioactive Constituents of Stephania abyssinica
Aims: To evaluate the hepatoprotective activity of Stephania abyssinica (S. abyssinica) and isolate the bioactive constituents. Study Design: The in vivo hepatoprotective activity of the extracts was evaluated by measuri...