Abstract

Background: Sex chromatin is a chromatin mass of 1 micron size usually seen at the periphery of nucleus in females. The term sex chromatin comprises of two superficially dissimilar structures known as Barr body in epithelial cells, other tissue cells and as Drumstick appearance in polymorphonuclear leucocytes. Aim of the study is gender determination by drumstick appearance of neutrophils using Leishman’s stain and Field stain. Materials and Methods: The present study included 60 individuals ranging from 20-50 age group of both the sexes to observe the morphology of sex chromatin in neutrophil by using Leishman’s and Field stain for the gender determination and comparison between the 2 staining procedure, using research microscope. Results: Presence of drumsticks, sessile nodule and non specific appendages like racket, tag, hook, minor lobe, small club, were found in neutrophils. In males 37% of drumstick, 7% of sessile nodule, 20% of racket structure, 3% small club, 13% minor lobe, 7% hook, 3% tag, was seen and in females 10% of drumstick, 60% of sessile nodule, 17% of racket structure, 7% small club, 1% minor lobe, 0% hook, 3% tag, was seen and field’s stain showed better results in appreciating the morphology of neutrophil. Conclusion: Observation on morphology and comparison between 2 different stain presented a moderate agreement in gender determination.

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