Gene Polymorphism of Matrix Metalloproteinase 9 in Asthenozoospermic Male Subjects
Journal Title: International Journal of Fertility & Sterility - Year 2018, Vol 11, Issue 4
Abstract
Background: Matrix metalloproteinase (MMPs) play important roles in the structural and functional properties of reproductive organs. The aim of this study is to determine the prevalence of C-1562T MMP-9 (rs3918242) gene polymorphism in fertile and infertile men. In addition, we aim to determine the association between C-1562T MMP-9 and G-1575A MMP-2 gene polymorphisms. Materials and Methods: A total of 400 subjects, including 200 fertile and 200 infertile men, were recruited for this casecontrol study. The allele frequencies and genotype distributions of single nucleotide polymorphism in the promoter regions of MMP-9 (C-1562T) were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The chi-square (χ2) test was used to assess the distribution of genotype frequencies. Results: There were no significant differences found in the genotype distributions or allele frequencies between fertile and infertile men for the C-1562T MMP-9 gene polymorphism. The percent of immotile sperm in infertile men with the CC and CT genotypes of C-1562T MMP-9 gene polymorphism significantly differed compared with that of subjects with the TT genotype. The frequency of CC/GA-combined genotypes of C-1562T MMP-9 and G-1575A MMP-2 gene polymorphisms significantly differed in fertile and infertile men (P=0.031). Conclusion: Our results suggest that genetic polymorphisms in MMP may impact male fertility
Authors and Affiliations
Heidar Tavilani
Keywords
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- EP ID EP561967
- DOI 10.22074/ijfs.2018.5038
- Views 139
- Downloads 0
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